IDO Protein (AA 2-403) (His tag)

Produktnummer ABIN6239816

Produktdetails

Target: IDO (Indoleamine 2,3-Dioxygenase (IDO))

Biologische Aktivität: Active

Proteineigenschaft: AA 2-403

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses IDO Protein ist gelabelt mit His tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 97 %

Biological Activity Comment: IDO (Indoleamine 2,3-dioxygenase 1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. Thus, bioactivity of recombinant human IDO was measured through its ability to oxidize L-tryptophan to N-formyl-kynurenine, using Methylene Blue as indicator. Briefly, a mixture of 800μM L-tryptophan, 9000 units/mL catalase, and 40μM Methyene Blue with the addition of 80mM ascorbic acid reaction buffer was incubated with different concentrations of IDO, and the absorbance was read in 321nm every 1.5h. The result indicated that recombinant human IDO oxidize L-tryptophan Enzyme activity of recombinant human IDO.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 6.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % skl, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: IDO (Indoleamine 2,3-Dioxygenase (IDO))

Andere Bezeichnung: Indoleamine-2,3-Dioxygenase (IDO) (IDO Produkte)

Synonyme: IDO Protein, IDO-1 Protein, INDO Protein, Ido Protein, Indo Protein, indoleamine 2,3-dioxygenase 1 Protein, IDO1 Protein, Ido1 Protein

Hintergrund: Alternative Names: CD107B, INDO, Indoleamine-Pyrrole 2,3 Dioxygenase

Molekulargewicht: 43kDa

UniProt: P14902