LDLR Protein (AA 22-788) (His tag,AVI tag,Biotin)

Produktnummer ABIN5954921

Produktdetails

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 22-788

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses LDLR Protein ist gelabelt mit His tag,AVI tag,Biotin.

Sequenz: AA 22-788

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Reinheit: >80 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Kommentare:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Antigendetails

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Andere Bezeichnung: LDL R (LDLR Produkte)

Synonyme: FH Protein, FHC Protein, LDLCQ2 Protein, Hlb301 Protein, LDLRA Protein, LDLA Protein, LDL receptor-2 Protein, fhc Protein, ldlcq2 Protein, ldlr Protein, ldlr2-a Protein, LDL receptor 1 Protein, ldlr-a Protein, ldlr-b Protein, low density lipoprotein receptor Protein, low density lipoprotein receptor a Protein, low density lipoprotein receptor S homeolog Protein, low density lipoprotein receptor L homeolog Protein, LDLR Protein, Ldlr Protein, ldlra Protein, ldlr.S Protein, ldlr.L Protein

Hintergrund: Low-Density Lipoprotein (LDL) Receptor is also known as LDLR, FH, FHC, LDLCQ2, and is a mosaic protein of ~840 amino acids (after removal of signal peptide) that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). It belongs to the Low density lipoprotein receptor gene family. LDL receptor complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell. This allows LDL-cholesterol to be bound and internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface. This occurs in all nucleated cells (not erythrocytes), but mainly in the liver which removes ~70 % of LDL from the circulation. Synthesis of receptors in the cell is regulated by the level of free intracellular cholesterol, if it is in excess for the needs of the cell then the transcription of the receptor gene will be inhibited. LDL receptors are translated by ribosomes on the endoplasmic reticulum and are modified by the Golgi apparatus before travelling in vesicles to the cell surface. LDL is directly involved in the development of atherosclerosis, due to accumulation of LDL-cholesterol in the blood. Atherosclerosis is the process responsible for the majority of cardiovascular diseases.

Molekulargewicht: 88.5 kDa

NCBI Accession: NP_000518

Pathways: Hepatitis C, Lipid Metabolism