CD74 Protein (AA 73-232) (His tag)

Produktnummer ABIN5854298

Produktdetails

Target: CD74 (HLA-DR-gamma (CD74))

Protein-Typ: Recombinant

Proteineigenschaft: AA 73-232

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses CD74 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: QQQGRLDKLT VTSQNLQLEN LRMKLPKPPK PVSKMRMATP LLMQALPMGA LPQGPMQNAT KYGNMTEDHV MHLLQNADPL KVYPPLKGSF PENLRHLKNT METIDWKVFE SWMHHWLLFE MSRHSLEQKP TDAPPKESLE LEDPSSGLGV TKQDLGPVPM HHHHHH

Reinheit: > 85 % by SDS - PAGE.

Endotoxin-Niveau: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Liquid. In phosphate buffered saline (  pH 7.4) containing 10 % glycerol.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: CD74 (HLA-DR-gamma (CD74))

Andere Bezeichnung: CD74 (CD74 Produkte)

Synonyme: DHLAG Protein, HLADG Protein, II Protein, Ia-GAMMA Protein, CLIP Protein, Ii Protein, INVG34 Protein, Iclp-1 Protein, iclp1 Protein, wu:fb11f09 Protein, wu:fb12a01 Protein, wu:fb13d06 Protein, wu:fk94e07 Protein, Iclp-2 Protein, cd74 Protein, iclp2 Protein, zgc:123161 Protein, CD74 molecule Protein, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) Protein, CD74 molecule, major histocompatibility complex, class II invariant chain a Protein, CD74 molecule, major histocompatibility complex, class II invariant chain b Protein, CD74 Protein, Cd74 Protein, cd74a Protein, cd74b Protein

Hintergrund: CD74, also known as HLA class II histocompatibility antigen gamma chain or HLA-DR antigens-associated invariant chain or CD74 (Cluster of Differentiation 74). The nascent MHC class II protein in the rough ER has its peptide-binding cleft blocked by the invariant chain (Ii, a trimer) to prevent it from binding cellular peptides or peptides from the endogenous pathway. The invariant chain also facilitates MHC class II's export from the ER in a vesicle. The signal for endosomal targeting resides in the cytoplasmic tail of the invariant chain. This fuses with a lateendosome containing the endocytosed, degraded proteins. It is then cleaved by cathepsin S (cathepsin L in cortical thymic epithelial cells), leaving only a small fragment calle CLIP which blocks peptide binding until HLA-DM binds to MHC II, releasing CLIP and allowing other peptides to bind. The stable MHC class-II is then presented on the cell surface. Recombinant human CD74, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molekulargewicht: 19.05kDa (166aa) 18-28kDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_004346

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints