PELI2 Protein (AA 1-420) (His tag)

Produktnummer ABIN5853851

Produktdetails

Target: PELI2 (Pellino Homolog 2 (Drosophila) (PELI2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-420

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PELI2 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSMFSPGQE EHCAPNKEPV KYGELVVLGY NGALPNGDRG RRKSRFALYK RPKANGVKPS TVHVISTPQA SKAISCKGQH SISYTLSRNQ TVVVEYTHDK DTDMFQVGRS TESPIDFVVT DTISGSQNTD EAQITQSTIS RFACRIVCDR NEPYTARIFA AGFDSSKNIF LGEKAAKWKN PDGHMDGLTT NGVLVMHPRG GFTEESQPGV WREISVCGDV YTLRETRSAQ QRGKLVESET NVLQDGSLID LCGATLLWRT ADGLFHTPTQ KHIEALRQEI NAARPQCPVG LNTLAFPSIN RKEVVEEKQP WAYLSCGHVH GYHNWGHRSD TEANERECPM CRTVGPYVPL WLGCEAGFYV DAGPPTHAFT PCGHVCSEKS AKYWSQIPLP HGTHAFHAAC PFCATQLVGE QNCIKLIFQG PID

Reinheit: > 80 % by SDS - PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1  mM DTT

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PELI2 (Pellino Homolog 2 (Drosophila) (PELI2))

Andere Bezeichnung: PELI2 (PELI2 Produkte)

Synonyme: AW047589 Protein, BB129927 Protein, pellino 2 Protein, pellino E3 ubiquitin protein ligase family member 2 Protein, Peli2 Protein, PELI2 Protein

Hintergrund: PELI2 is an E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. This protein is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. It mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. PELI2 may be important for LPS- and IL1B-induced MAP3K7-dependent, but not MAP3K3-dependent, NF-kappa-B activation. It can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1. Recombinant human PELI2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 48.8 kDa (443aa) confirmed by MALDI-TOF

NCBI Accession: NP_067078

UniProt: Q9HAT8

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades