POLR2F Protein (AA 1-127) (His tag)
-
- Target Alle POLR2F Proteine anzeigen
- POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-127
-
Spezies
- Human
-
Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses POLR2F Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- MGSSHHHHHH SSGLVPRGSH MGSMSDNEDN FDGDDFDDVE EDEGLDDLEN AEEEGQENVE ILPSGERPQA NQKRITTPYM TKYERARVLG TRALQIAMCA PVMVELEGET DPLLIAMKEL KARKIPIIIR RYLPDGSYED WGVDELIITD
- Reinheit
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product POLR2F Protein
-
Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 1-127) protein (His tag)
POLR2F Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 1-127) protein (GST tag)POLR2F Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 28-127) protein (GST tag)POLR2F Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 2-127) protein (His tag)Crystallography grade POLR2F Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 2-127) protein (His tag)Crystallography grade POLR2F Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 2-216), (partial) protein (GST tag)POLR2F Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant 90 % ELISA
Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) protein (Myc-DYKDDDDK Tag)POLR2F Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 1.0 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- Target
- POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))
- Andere Bezeichnung
- POLR2F (POLR2F Produkte)
- Synonyme
- HRBP14.4 Protein, POLRF Protein, RPABC2 Protein, RPB14.4 Protein, RPB6 Protein, 1810060D16Rik Protein, RNA polymerase II subunit F Protein, polymerase (RNA) II (DNA directed) polypeptide F Protein, POLR2F Protein, Polr2f Protein
- Hintergrund
- POLR2F is the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Recombinant human POLR2F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molekulargewicht
- 16.9kDa (150aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
- NCBI Accession
- NP_068809
- UniProt
- P61218
- Pathways
- Regulatorische RNA Pathways
-
