POLR2F Protein (AA 2-216, partial) (GST tag)

Produktnummer ABIN1046925

Produktdetails

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-216, partial

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses POLR2F Protein ist gelabelt mit GST tag.

Applikation: ELISA

Sequenz: MLGTEGGEGF VVKVRGLPWS CSADEVQRFF SDCKIQNGAQ GIRFIYTREG RPSGEAFVEL ESEDEVKLAL KKDRETMGHR YVEVFKSNNV EMDWVLKHTG PNSPDTANDG FVRLRGLPFG CSKEEIVQFF SGLEIVPNGI TLPVDFQGRS TGEAFVQFAS QEIAEKALKK HKERIGHRYI EIFKSSRAEV RTHYDPPRKL MAMQRPGPYD RPGAG

Produktmerkmale: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Reinheit: 90 %

Anwendungsinformationen

Kommentare:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handhabung: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Referenzen

Pusch, Wang, Roe, Blin: "Genomic structure of the RNA polymerase II small subunit (hRPB14.4) locus (POLRF) and mapping to 22q13.1 by sequence identity." in: Genomics, Vol. 34, Issue 3, pp. 440-2, (1996) (PubMed).

Acker, Wintzerith, Vigneron, Kedinger: "A 14.4 KDa acidic subunit of human RNA polymerase II with a putative leucine-zipper." in: DNA sequence : the journal of DNA sequencing and mapping, Vol. 4, Issue 5, pp. 329-31, (1995) (PubMed).

Antigendetails

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Andere Bezeichnung: DNA-directed RNA polymerases I, II, and III subunit RPABC2 protein (POLR2F Produkte)

Synonyme: HRBP14.4 Protein, POLRF Protein, RPABC2 Protein, RPB14.4 Protein, RPB6 Protein, 1810060D16Rik Protein, RNA polymerase II subunit F Protein, polymerase (RNA) II (DNA directed) polypeptide F Protein, POLR2F Protein, Polr2f Protein

Hintergrund: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and togther with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds.

Molekulargewicht: 51.6 kD

UniProt: P31943

Pathways: Regulatorische RNA Pathways