MCM7 Protein (AA 1-414) (His tag)

Produktnummer ABIN5853499

Produktdetails

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-414

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses MCM7 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSMVVATYT CDQCGAETYQ PIQSPTFMPL IMCPSQECQT NRSGGRLYLQ TRGSRFIKFQ EMKMQEHSDQ VPVGNIPRSI TVLVEGENTR IAQPGDHVSV TGIFLPILRT GFRQVVQGLL SETYLEAHRI VKMNKSEDDE SGAGELTREE LRQIAEEDFY EKLAASIAPE IYGHEDVKKA LLLLLVGGVD QSPRGMKIRG NINICLMGDP GVAKSQLLSY IDRLAPRSQY TTGRGSSGVG LTAAVLRDSV SGELTLEGGA LVLADQGVCC IDEFDKMAEA DRTAIHEVME QQTISIAKAG ILTTLNARCS ILAAANPAYG RYNPRRSLEQ NIQLPAALLS RFDLLWLIQD RPDRDNDLRL AQHITYVHQH SRQPPSQFEP LDMKLMRRYI AMCREKQPMV PESLADYITA AYVEMRR

Reinheit: > 85 % by SDS - PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Denatured

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.4M uREA, 10 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Andere Bezeichnung: MCM7 (MCM7 Produkte)

Synonyme: CDC47 Protein, MCM2 Protein, P1.1-MCM3 Protein, P1CDC47 Protein, P85MCM Protein, PNAS146 Protein, MCM7 Protein, AI747533 Protein, Mcmd7 Protein, mCDC47 Protein, chunp6911 Protein, nyz175 Protein, sr:nyz175 Protein, cdc47 Protein, cdc47-2 Protein, mcm7 Protein, xmcm7 Protein, CG4978 Protein, DmMCM7 Protein, DmeMCM7 Protein, Dmel\\CG4978 Protein, McM7 Protein, Mcp-PCR1 Protein, PCR1 Protein, anon-EST:Liang-1.66 Protein, clone 1.66 Protein, minichromosome maintenance complex component 7 Protein, mini-chromosome maintenance complex protein 7 Protein, minichromosome maintenance complex component 2 Protein, minichromosome maintenance complex component 7 L homeolog Protein, Minichromosome maintenance 7 Protein, minichromosome maintenance complex component 7 S homeolog Protein, MCM complex subunit Mcm7 Protein, MCM7 Protein, CDC47 Protein, Mcm7 Protein, MCM2 Protein, mcm7 Protein, mcm7.L Protein, mcm7.S Protein

Hintergrund: MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Recombinant human MCM7 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molekulargewicht: 48.6kDa (437aa)

NCBI Accession: NP_877577

UniProt: P33993

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA