EIF3F Protein (AA 1-357) (His tag)

Produktnummer ABIN5853278

Produktdetails

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-357

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses EIF3F Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSMATPAVP VSAPPATPTP VPAAAPASVP APTPAPAAAP VPAAAPASSS DPAAAAAATA APGQTPASAQ APAQTPAPAL PGPALPGPFP GGRVVRLHPV ILASIVDSYE RRNEGAARVI GTLLGTVDKH SVEVTNCFSV PHNESEDEVA VDMEFAKNMY ELHKKVSPNE LILGWYATGH DITEHSVLIH EYYSREAPNP IHLTVDTSLQ NGRMSIKAYV STLMGVPGRT MGVMFTPLTV KYAYYDTERI GVDLIMKTCF SPNRVIGLSS DLQQVGGASA RIQDALSTVL QYAEDVLSGK VSADNTVGRF LMSLVNQVPK IVPDDFETML NSNINDLLMV TYLANLTQSQ IALNEKLVNL

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Denatured

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 10 % glycerol, 0.4M urea

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Andere Bezeichnung: EIF3F (EIF3F Produkte)

Synonyme: AO090005001590 Protein, Dmel\CG9769 Protein, eIF3-S5 Protein, eif3f Protein, LOC733116 Protein, eIF3f Protein, EIF3S5 Protein, eIF3-p47 Protein, 0610037M02Rik Protein, AA409853 Protein, Eif3s5 Protein, eukaryotic translation initiation factor 3 subunit F Protein, hypothetical protein Protein, CG9769 gene product from transcript CG9769-RA Protein, eukaryotic translation initiation factor 3 subunit 5 Protein, eukaryotic translation initiation factor 3, subunit F Protein, Eukaryotic translation initiation factor 3 subunit F Protein, eif3f Protein, ANI_1_120074 Protein, AOR_1_2780174 Protein, PTRG_03897 Protein, PAAG_00376 Protein, MCYG_08502 Protein, MGYG_08653 Protein, PGTG_04587 Protein, CG9769 Protein, LOC733116 Protein, EIF3F Protein, Eif3f Protein, eif-3.F Protein

Hintergrund: EIF3F is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF 2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AuG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Recombinant human EIF3F protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molekulargewicht: 40.0kDa (380aa)

NCBI Accession: NP_003745

UniProt: O00303

Pathways: Ribonucleoprotein Complex Subunit Organization