EIF3F Protein (AA 2-250, partial) (GST tag)

Produktnummer ABIN1046992

Produktdetails

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-250, partial

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses EIF3F Protein ist gelabelt mit GST tag.

Applikation: ELISA

Sequenz: ATPAVPVSAP PATPTPVPAA APASVPAPTP APAAAPVPAA APASSSDPAA AAAATAAPGQ TPASAQAPAQ TPAPALPGPA LPGPFPGGRV VRLHPVILAS IVDSYERRNE GAARVIGTLL GTVDKHSVEV TNCFSVPHNE SEDEVAVDME FAKNMYELHK KVSPNELILG WYATGHDITE HSVLIHEYYS REAPNPIHLT VDTSLQNGRM SIKAYVSTLM GVPGRTMGVM FTPLTVKYAY YDTERIGVD

Produktmerkmale: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Reinheit: 90 %

Anwendungsinformationen

Kommentare:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handhabung: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Referenzen

Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, (2003) (PubMed).

Asano, Vornlocher, Richter-Cook, Merrick, Hinnebusch, Hershey: "Structure of cDNAs encoding human eukaryotic initiation factor 3 subunits. Possible roles in RNA binding and macromolecular assembly." in: The Journal of biological chemistry, Vol. 272, Issue 43, pp. 27042-52, (1997) (PubMed).

Antigendetails

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Andere Bezeichnung: Eukaryotic translation initiation factor 3 subunit F protein (EIF3F Produkte)

Synonyme: AO090005001590 Protein, Dmel\CG9769 Protein, eIF3-S5 Protein, eif3f Protein, LOC733116 Protein, eIF3f Protein, EIF3S5 Protein, eIF3-p47 Protein, 0610037M02Rik Protein, AA409853 Protein, Eif3s5 Protein, eukaryotic translation initiation factor 3 subunit F Protein, hypothetical protein Protein, CG9769 gene product from transcript CG9769-RA Protein, eukaryotic translation initiation factor 3 subunit 5 Protein, eukaryotic translation initiation factor 3, subunit F Protein, Eukaryotic translation initiation factor 3 subunit F Protein, eif3f Protein, ANI_1_120074 Protein, AOR_1_2780174 Protein, PTRG_03897 Protein, PAAG_00376 Protein, MCYG_08502 Protein, MGYG_08653 Protein, PGTG_04587 Protein, CG9769 Protein, LOC733116 Protein, EIF3F Protein, Eif3f Protein, eif-3.F Protein

Hintergrund: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Molekulargewicht: 53.2 kD

UniProt: O00303

Pathways: Ribonucleoprotein Complex Subunit Organization