SET/TAF-I Protein (AA 1-290) (His tag)

Produktnummer ABIN5853066

Produktdetails

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-290

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses SET/TAF-I Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSMAPKRQS PLPPQKKKPR PPPALGPEET SASAGLPKKG EKEQQEAIEH IDEVQNEIDR LNEQASEEIL KVEQKYNKLR QPFFQKRSEL IAKIPNFWVT TFVNHPQVSA LLGEEDEEAL HYLTRVEVTE FEDIKSGYRI DFYFDENPYF ENKVLSKEFH LNESGDPSSK STEIKWKSGK DLTKRSSQTQ NKASRKRQHE EPESFFTWFT DHSDAGADEL GEVIKDDIWP NPLQYYLVPD MDDEEGEGEE DDDDDEEEEG LEDIDEEGDE DEGEEDEDDD EGEEGEEDEG EDD

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1  mM DTT.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Andere Bezeichnung: SET (SET Produkte)

Synonyme: 2PP2A Protein, I2PP2A Protein, IGAAD Protein, IPP2A2 Protein, PHAPII Protein, TAF-I Protein, TAF-IBETA Protein, 2610030F17Rik Protein, 5730420M11Rik Protein, AA407739 Protein, I-2PP2A Protein, StF-IT-1 Protein, 2pp2a Protein, i2pp2a Protein, igaad Protein, ipp2a2 Protein, phapii Protein, set Protein, set-a Protein, set-b Protein, taf-ibeta Protein, wu:fb30g11 Protein, wu:fd16c08 Protein, SET nuclear proto-oncogene Protein, SET nuclear oncogene Protein, SET translocation Protein, SET nuclear proto-oncogene L homeolog Protein, SET nuclear proto-oncogene b Protein, SET Protein, Set Protein, LOC403555 Protein, set.L Protein, setb Protein

Hintergrund: SET inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. Recombinant human SET protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 35.9 kDa (313aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)

NCBI Accession: NP_001116293

UniProt: Q01105

Pathways: Apoptose