RPA1 Protein (AA 2-616) (His-SUMO Tag)

Produktnummer ABIN5710272

Produktdetails

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-616

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses RPA1 Protein ist gelabelt mit His-SUMO Tag.

Applikation: SDS-PAGE (SDS)

Sequenz: VGQLSEGAIA AIMQKGDTNI KPILQVINIR PITTGNSPPR YRLLMSDGLN TLSSFMLATQ LNPLVEEEQL SSNCVCQIHR FIVNTLKDGR RVVILMELEV LKSAEAVGVK IGNPVPYNEG LGQPQVAPPA PAASPAASSR PQPQNGSSGM GSTVSKAYGA SKTFGKAAGP SLSHTSGGTQ SKVVPIASLT PYQSKWTICA RVTNKSQIRT WSNSRGEGKL FSLELVDESG EIRATAFNEQ VDKFFPLIEV NKVYYFSKGT LKIANKQFTA VKNDYEMTFN NETSVMPCED DHHLPTVQFD FTGIDDLENK SKDSLVDIIG ICKSYEDATK ITVRSNNREV AKRNIYLMDT SGKVVTATLW GEDADKFDGS RQPVLAIKGA RVSDFGGRSL SVLSSSTIIA NPDIPEAYKL RGWFDAEGQA LDGVSISDLK SGGVGGSNTN WKTLYEVKSE NLGQGDKPDY FSSVATVVYL RKENCMYQAC PTQDCNKKVI DQQNGLYRCE KCDTEFPNFK YRMILSVNIA DFQENQWVTC FQESAEAILG QNAAYLGELK DKNEQAFEEV FQNANFRSFI FRVRVKVETY NDESRIKATV MDVKPVDYRE YGRRLVMSIR RSALM

Aufreinigung: SDS-PAGE

Reinheit: > 90 %

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Lagerung: -80 °C,4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Antigendetails

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Andere Bezeichnung: RFA1 (RPA1 Produkte)

Synonyme: HSSB Protein, MST075 Protein, REPA1 Protein, RF-A Protein, RP-A Protein, RPA70 Protein, Cb1-727 Protein, wu:fi14b08 Protein, zgc:55337 Protein, zgc:77092 Protein, RPA1 Protein, hssb Protein, mst075 Protein, repa1 Protein, rf-a Protein, rp-a Protein, rpa Protein, rpa70 Protein, LOC100230990 Protein, 5031405K23Rik Protein, 70kDa Protein, AA589576 Protein, AW557552 Protein, Rpa Protein, CG9633 Protein, D-RPA70 Protein, D-SSB Protein, DRP-A Protein, DmRPA Protein, Dmel\\CG9633 Protein, RPA Protein, RPA 70 Protein, RPA 70 kDa Protein, RpA70 Protein, Rpa-70 Protein, Ssb-70 Protein, dRP-A Protein, dmrpa1 Protein, i164 Protein, replication protein A1 Protein, replication protein A1, 70kDa Protein, replication protein A1 L homeolog Protein, Replication Protein A 70 Protein, RPA1 Protein, Rpa1 Protein, rpa1 Protein, rpa1.L Protein, RpA-70 Protein

Hintergrund: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage . In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response . It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage . Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair . Plays also a role in base excision repair (BER) probably through interaction with UNG . Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance . As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange

Molekulargewicht: 84 kDa

UniProt: P27694

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA