ITGAV/ITGB6 Protein (AA 31-992) (His tag,AVI tag,Biotin)

Produktnummer ABIN5674599

Produktdetails

Target: ITGAV/ITGB6 (Integrin AlphaV Beta6 (ITGAV/ITGB6))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 31-992

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses ITGAV/ITGB6 Protein ist gelabelt mit His tag,AVI tag,Biotin.

Marke: PrecisionAvi

Sequenz: AA 31-992

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Produktmerkmale: Biotinylated Human ITGAV & ITGB6 Heterodimer Protein, produced by co-expression of ITGAV and ITGB6, has a calculated MW of 113 kDa (ITGAV) and 79.6 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine

Reinheit: >90 % as determined by reduced SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Kommentare:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 50 mM Tris, 150 mM NaCl, pH 7.5

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Antigendetails

Target: ITGAV/ITGB6 (Integrin AlphaV Beta6 (ITGAV/ITGB6))

Andere Bezeichnung: Integrin alpha V beta 6 (ITGAV/ITGB6 Produkte)

Hintergrund: Integrin alpha V beta 6 is a heterodimer of beta-6 associating with alpha-V. Integrin alpha-V beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. Also, Integrin alpha-V beta-6 acts as a receptor for coxsackievirus A9 and coxsackievirus B1 as well as herpes simplex virus-1/HHV-1. Furthermore, it binds the TGF-beta latency-associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes. This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1.

Molekulargewicht: 114.7 kDa (ITGAV) & 79.6 kDa (ITGB6)