ITGAV/ITGB5 Protein (AA 31-992) (His tag)

Produktnummer ABIN4949118

Produktdetails

Target: ITGAV/ITGB5 (Integrin alpha V / beta 5 (ITGAV/ITGB5))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 31-992

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses ITGAV/ITGB5 Protein ist gelabelt mit His tag.

Applikation: Functional Studies (Func)

Sequenz: AA 31-992

Produktmerkmale: Human ITGAV & ITGB5 Heterodimer Protein, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 113 kDa (ITGAV) and 81.9 kDa (ITGB5). Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB5 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 145 kDa (ITGAV) and 100-116 kDa (ITGB5) respectively due to glycosylation.

Reinheit: >95 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 50 mM Tris, 150 mM NaCl, pH 7.5

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Antigendetails

Target: ITGAV/ITGB5 (Integrin alpha V / beta 5 (ITGAV/ITGB5))

Andere Bezeichnung: Integrin alpha V beta 5 (ITGAV/ITGB5 Produkte)

Hintergrund: Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.

Molekulargewicht: 112.9 kDa (ITGAV) & 81.9 kDa (ITGB5)