WNT5A Protein (AA 38-380)

Produktnummer ABIN4948753

Produktdetails

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 38-380

Spezies: Maus, Human

Quelle: Mammalian Cells

Applikation: Functional Studies (Func)

Spezifität: Stem cell or organoid culture

Aufreinigung: This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.

Reinheit: 70-75 %

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg)

Biological Activity Comment: The inhibitory activity of Wnt5a on the canonical Wnt pathway has been measured using TCF-based Wnt reporter stable cell line stimulated by mouse Wnt3a. IE50 of Wnt5a is about 50 - 100 ng/mL in the presence of 1 -2 ng/mL of mouse Wnt3a.

Anwendungsinformationen

Applikationshinweise: 100 ng/mL
To treat cell lines, dilute the protein solution at least 200 times in medium, to treat stem cells, dilute the protein solution at least 500 times in medium. Diluted Wnt proteins in medium or phosphate buffer can be stored at 4 °C for few days only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Phosphate buffer pH 7.4-7.6, 1 % CHAPS, 0.1 % BSA

Handhabung: Mix the protein by pipetting up and down but not by vortexing

Lagerung: -20 °C

Informationen zur Lagerung: Keep the protein frozen until use. Refreeze aliquots at -20C or below but avoid freeze-thaw circles.

Antigendetails

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Andere Bezeichnung: Wnt5a (WNT5A Produkte)

Synonyme: hWNT5A Protein, wnt-5A Protein, wnt5a-B Protein, WNT5A Protein, 8030457G12Rik Protein, Wnt-5a Protein, Wnt family member 5A Protein, Wnt family member 5A S homeolog Protein, wingless-type MMTV integration site family, member 5a Protein, wingless-type MMTV integration site family, member 5A Protein, wingless-related MMTV integration site 5A Protein, Wnt signaling ligand Protein, WNT5A Protein, Wnt5a Protein, wnt5a.S Protein, wnt5a Protein, LOC578814 Protein, wnt5 Protein

Hintergrund: The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. Protein Wnt5a is a protein that is encoded by the WNT5A gene. This gene is a member of the noncanonical WNT gene family. Mature mouse Wnt5a is 100 % identical in amino acids to mature human Wnt5a after being secreted from expression cells. Wnt5a protein can inhibit or activate TCF-based Wnt signaling.

Molekulargewicht: 45 kDa evaluated by SDS-PAGE under reducing conditions

Gen-ID: 7474, 22418

NCBI Accession: NC_000080

UniProt: P41221, P22725

Pathways: WNT Signalweg, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation