Glutamate Receptor 3 Protein (AA 23-888) (rho-1D4 tag)

Produktnummer ABIN3137647

Produktdetails

Target: Glutamate Receptor 3 (GRIA3) (Glutamate Receptor, Ionotropic, AMPA 3 (GRIA3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 23-888

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses Glutamate Receptor 3 Protein ist gelabelt mit rho-1D4 tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: GFPNTISIGG LFMRNTVQEH SAFRFAVQLY NTNQNTTEKP FHLNYHVDHL DSSNSFSVTN AFCSQFSRGV YAIFGFYDQM SMNTLTSFCG ALHTSFVTPS FPTDADVQFV IQMRPALKGA ILSLLGYYKW EKFVYLYDTE RGFSILQAIM EAAVQNNWQV TARSVGNIKD IQEFRRIIEE MDRRQEKRYL IDCEVERINT ILEQVVILGK HSRGYHYMLA NLGFTDIVLE RVMHGGANIT GFQIVNNENP MVQQFIQRWV RLDEREFPEA KNAPLKYTSA LTHDAILVIA EAFRYLRRQR VDVSRRGSAG DCLANPAVPW SQGIDIERAL KMVQVQGMTG NIQFDTYGRR TNYTIDVYEM KVSGSRKAGY WNEYERFVPF SDQQISNDSS SSENRTIVVT TILESPYVMY KKNHEQLEGN ERYEGYCVDL AYEIAKHVRI KYKLSIVGDG KYGARDPETK IWNGMVGELV YGRADIAVAP LTITLVREEV IDFSKPFMSL GISIMIKKPQ KSKPGVFSFL DPLAYEIWMC IVFAYIGVSV VLFLVSRFSP YEWHLEDNNE EPRDPQSPPD PPNEFGIFNS LWFSLGAFMQ QGCDISPRSL SGRIVGGVWW FFTLIIISSY TANLAAFLTV ERMVSPIESA EDLAKQTEIA YGTLDSGSTK EFFRRSKIAV YEKMWSYMKS AEPSVFTKTT ADGVARVRKS KGKFAFLLES TMNEYIEQRK PCDTMKVGGN LDSKGYGVAT PKGSALRTPV NLAVLKLSEQ GILDKLKNKW WYDKGECGAK DSGSKDKTSA LSLSNVAGVF YILVGGLGLA MMVALIEFCY KSRAESKRMK LTKNTQNFKP APATNTQNYA TYREGYNVYG TESVKI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Gria3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Glutamate Receptor 3 (GRIA3) (Glutamate Receptor, Ionotropic, AMPA 3 (GRIA3))

Andere Bezeichnung: Gria3 (GRIA3 Produkte)

Synonyme: AMPA Protein, gria3 Protein, GRIN3A Protein, glur3b Protein, gria3.2 Protein, zfGRIA3[b] Protein, si:bz1o18.1 Protein, SI:zC231H1.1 Protein, si:zc140k5.3 Protein, GLUR3 Protein, GLUR-C Protein, GLUR3/C Protein, GRIA3 Protein, glur3 Protein, GluA3 Protein, GluR-3 Protein, GluR-C Protein, GluR-K3 Protein, GLUR-K3 Protein, GLURC Protein, MRX94 Protein, 2900064I19Rik Protein, Glur-3 Protein, Glur3 Protein, Gluralpha3 Protein, glutamate receptor, ionotropic, AMPA 3b Protein, glutamate ionotropic receptor AMPA type subunit 3 Protein, glutamate receptor, ionotropic, AMPA 3 Protein, glutamate receptor, ionotropic, AMPA 3 L homeolog Protein, glutamate receptor, ionotropic, AMPA3 (alpha 3) Protein, gria3b Protein, GRIA3 Protein, gria3 Protein, gria3.L Protein, Gria3 Protein

Hintergrund: Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate (By similarity). {ECO:0000250}.

Molekulargewicht: 99.4 kDa Including tag.

UniProt: Q9Z2W9

Pathways: PI3K-Akt Signalweg, cAMP Metabolic Process, Synaptic Membrane