ATP13A2 Protein (AA 1-1169) (Strep Tag)

Produktnummer ABIN3137038

Produktdetails

Target: ATP13A2 (ATPase Type 13A2 (ATP13A2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1169

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses ATP13A2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MSADSSLLMG STPPSYGTLT TGTSIDPLSS SASSVRLSGY CGSPWRAIGY HAAVWMLAGI PWLLFRWKPL WGVRLRLKPC SLAHAETLVI EIKDKEGSSR QLFTVQVQTE AVVQGSLELP PQAQAEDGRS QAAVGVTPEG TWQDTSELHR QEEAKQVLRY YVLQGQRYVW METQQAFCQV SLLDHGRTCD DVHCSSSGLS LQDQATRKTI YGPNVISIPV KSYLQLLADE ALNPYYGFQA FSIALWLADH YYWYALCIFL ISAISICLAL YKTRKQSLTL RDMVKLSVRV QVCRPGGEEE WVDSSELVPG DCLVLPQEGG VMPCDAALVA GECVVNESSL TGESTPVLKT ALPEGPKPYC PETHRRHTLF CGTLILQARA YVGPRVLAVV TRTGFCTAKG GLVSSILHPR PISFKFYKHS MKFVAALSVL ALLGTVYSII ILYRNRVPVR EIVIRALDLV TVVVPPALPA AMTVCTLYAQ SRLRTQGIFC IHPLRINLGG KLRLVCFDKT GTLTEDGLDV MGVVPLKGQV LLPLVPEPCH LPLGPLLRAL ATCHALSQLH DTPVGDPMDL KMVESTGWVL EEGPAAGSAP GSQVLVVMRP PPGGPRQQEE PPVPVSVLCR FPFSSALQRM DVVVTWPGAT QPEAYVKGSP ELVASLCSPE TVPSDFSQVL QSYTAAGYRV VALAGKPLPI APSLAAAQQL TRDTVERELS LLGLLVMRNL LKPQTAPVIQ TLRKTGIRTV MVTGDNLQTA VTVARACGMV GAQEHLAVIH ATHPEQGQPA ALEFLPTESS AVMNGAKATG YPTVPEPQSC HLALSGSTFA VLRKHFPKLL PKVLVQATVF ARMAPEQKTE LVCELQRLQY CVGMCGDGAN DCGALKAADV GISLSQAEAS VVSPFTSSMA SIECVPTVIR EGRCSLDTSF SVFKYMALYS LTQFISVLIL YTINTNLGDL QFLAIDLVIT TTVAVLMSRT GPALTLVRAR PPGALLSVPV LGSLLLQVAL VAGIQLGGYF LVIAQPWFVP LNRTVPAPDN LPNYENTVVF SLSGFQYLIL AAAVSKGAPF RQPLYTNVPF LVALALLGSV LVGLILVPGL LQGPLGLRNI VDSSFKLLLL GLVAFNFVGA FMLESVLDQC LPACLRWLRP KRASKKQFKR LQQELAEHPW PTLPVGSVR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ATP13A2 (ATPase Type 13A2 (ATP13A2))

Andere Bezeichnung: Atp13a2 (ATP13A2 Produkte)

Synonyme: ATP13A2 Protein, 1110012E06Rik Protein, AA589443 Protein, RGD1307977 Protein, im:7147302 Protein, zgc:136762 Protein, CLN12 Protein, HSA9947 Protein, KRPPD Protein, PARK9 Protein, ATPase 13A2 Protein, ATPase type 13A2 Protein, probable cation-transporting ATPase 13A2 Protein, ATP13A2 Protein, Atp13a2 Protein, atp13a2 Protein, PTRG_01071 Protein, MCYG_01937 Protein, LOC100439053 Protein

Hintergrund: Polyamine-transporting ATPase 13A2 (EC 7.6.2.-),FUNCTION: ATPase which acts as a lysosomal polyamine exporter with high affinity for spermine (By similarity). Also stimulates cellular uptake of polyamines and protects against polyamine toxicity (By similarity). Plays a role in intracellular cation homeostasis and the maintenance of neuronal integrity (By similarity). Contributes to cellular zinc homeostasis (By similarity). Confers cellular protection against Mn(2+) and Zn(2+) toxicity and mitochondrial stress (By similarity). Required for proper lysosomal and mitochondrial maintenance (By similarity). Regulates the autophagy-lysosome pathway through the control of SYT11 expression at both transcriptional and post-translational levels (PubMed:27278822). Facilitates recruitment of deacetylase HDAC6 to lysosomes to deacetylate CTTN, leading to actin polymerization, promotion of autophagosome-lysosome fusion and completion of autophagy (PubMed:30538141). Promotes secretion of exosomes as well as secretion of SCNA via exosomes (By similarity). Plays a role in lipid homeostasis (By similarity). {ECO:0000250|UniProtKB:Q9NQ11, ECO:0000269|PubMed:27278822, ECO:0000269|PubMed:30538141}.

Molekulargewicht: 126.4 kDa

UniProt: Q9CTG6

Pathways: Ribonucleoside Biosynthetic Process