KCNT1 Protein (AA 1-1224) (Strep Tag)

Produktnummer ABIN3135864

Produktdetails

Target: KCNT1 (Potassium Channel, Subfamily T, Member 1 (KCNT1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1224

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses KCNT1 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MARAKLPRSP SEGKAGPGDT PAGAAAPEEP HGLSPLLPAR GGGSVGSDVG QRVQVEFYVN ENTFKERLKL FFIKNQRSSL RIRLFNFSLK LLTCLLYIVR VLLDNPDQGI GCWGCTKYNY TFNGSSSEFH WAPILWVERK MALWVIQVIV ATISFLETML IIYLSYKGNI WEQIFHVSFV LEMINTLPFI ITVFWPPLRN LFIPVFLNCW LAKHALENMI NDFHRAILRT QSAMFNQVLI LFCTLLCLVF TGTCGIQHLE RAGGNLNLLT SFYFCIVTFS TVGFGDVTPK IWPSQLLVVI LICVTLVVLP LQFEELVYLW MERQKSGGNY SRHRARTEKH VVLCVSSLKI DLLMDFLNEF YAHPRLQDYY VVILCPSEMD VQVRRVLQIP LWSQRVIYLQ GSALKDQDLM RAKMDNGEAC FILSSRNEVD RTAADHQTIL RAWAVKDFAP NCPLYVQILK PENKFHVKFA DHVVCEEECK YAMLALNCIC PATSTLITLL VHTSRGQEGQ ESPEQWQRTY GRCSGNEVYH IRMGDSKFFR EYEGKSFTYA AFHAHKKYGV CLIGLKREEN KSILLNPGPR HILAASDTCF YINITKEENS AFIFKQEEKQ KRRGLAGQAL YEGPSRLPVH SIIASMGTVA MDLQNTDCRP SQGGSGGDGT KLTLPTENGS GSRRPSIAPV LELADSSALL PCDLLSDQSE DEVTPSDDEG LSVVEYVKGY PPNSPYIGSS PTLCHLLPVK APFCCLRLDK GCKHNSYEDA KAYGFKNKLI IVSAETAGNG LYNFIVPLRA YYRSRRELNP IVLLLDNKPD HHFLEAICCF PMVYYMEGSV DNLDSLLQCG IIYADNLVVV DKESTMSAEE DYMADAKTIV NVQTMFRLFP SLSITTELTH PSNMRFMQFR AKDSYSLALS KLEKQERENG SNLAFMFRLP FAAGRVFSIS MLDTLLYQSF VKDYMITITR LLLGLDTTPG SGYLCAMKVT EDDLWIRTYG RLFQKLCSSS AEIPIGIYRT ECHVFSEPHD VRAQSQISVN MEDCEDTREA KGPWGTRAAS GSGSTHGRHG GSADPVEHPL LRRKSLQWAR KLSRKSTKQA GKAPVATDWI TQQRLSLYRR SERQELSELV KNRMKHLGLP TTGYEDVANL TASDVMNRVN LGYLQDEMND HHQNTLSYVL INPPPDTRLE PNDIVYLIRS DPLAHVASSS QSRKSSCSNK LSSCNPETRD ETQL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: KCNT1 (Potassium Channel, Subfamily T, Member 1 (KCNT1))

Andere Bezeichnung: Kcnt1 (KCNT1 Produkte)

Synonyme: EIEE14 Protein, ENFL5 Protein, KCa4.1 Protein, SLACK Protein, bA100C15.2 Protein, Slack Protein, rSlo2 Protein, C030030G16Rik Protein, mKIAA1422 Protein, slo2 Protein, slack Protein, Slo2.2 Protein, MGC146594 Protein, potassium sodium-activated channel subfamily T member 1 Protein, potassium channel subfamily T member 1 Protein, potassium channel, subfamily T, member 1 Protein, potassium channel, sodium activated subfamily T, member 1 Protein, KCNT1 Protein, LOC100436526 Protein, Kcnt1 Protein, kcnt1 Protein

Hintergrund: Potassium channel subfamily T member 1,FUNCTION: Outwardly rectifying potassium channel subunit that may coassemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro) (By similarity). {ECO:0000250}.

Molekulargewicht: 138.1 kDa

UniProt: Q6ZPR4