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ANGPTL3 Protein (Angiopoietin-Like 3) (AA 17-455) (His tag)

Crystallography grade ANGPTL3 Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3137445
$6,086.30
Zzgl. Versandkosten $45.00 und $20.00 Trockeneis
Lieferung in 30 bis 35 Werktagen
  • Target Alle ANGPTL3 Proteine anzeigen
    ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 17-455
    Spezies
    • 17
    • 5
    • 2
    Maus
    Quelle
    • 11
    • 7
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses ANGPTL3 Protein ist gelabelt mit His tag.
    Applikation
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    SRVDPDLSSF DSAPSEPKSR FAMLDDVKIL ANGLLQLGHG LKDFVHKTKG QINDIFQKLN IFDQSFYDLS LRTNEIKEEE KELRRTTSTL QVKNEEVKNM SVELNSKLES LLEEKTALQH KVRALEEQLT NLILSPAGAQ EHPEVTSLKS FVEQQDNSIR ELLQSVEEQY KQLSQQHMQI KEIEKQLRKT GIQEPSENSL SSKSRAPRTT PPLQLNETEN TEQDDLPADC SAVYNRGEHT SGVYTIKPRN SQGFNVYCDT QSGSPWTLIQ HRKDGSQDFN ETWENYEKGF GRLDGEFWLG LEKIYAIVQQ SNYILRLELQ DWKDSKHYVE YSFHLGSHET NYTLHVAEIA GNIPGALPEH TDLMFSTWNH RAKGQLYCPE SYSGGWWWND ICGENNLNGK YNKPRTKSRP ERRRGIYWRP QSRKLYAIKS SKMMLQPTT
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Angptl3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))
    Andere Bezeichnung
    Angptl3 (ANGPTL3 Produkte)
    Synonyme
    fb60e11, fb66h02, wu:fb60e11, wu:fb66h02, zgc:111943, ANGPTL3, LOC100230785, ANG-5, ANGPT5, ANL3, FHBL2, hypl, angiopoietin-like 3, angiopoietin like 3, angptl3, ANGPTL3, Angptl3
    Hintergrund
    Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism (PubMed:11788823, PubMed:12671033). Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake (PubMed:26305978). Has a stimulatory effect on plasma triglycerides (TG), which is achieved by suppressing plasma TG clearance via inhibition of LPL activity, the function seems to be specific for the feeding conditions. The inhibition of LPL activity appears to be an indirect mechanism involving recruitment of proprotein convertases PCSK6 and FURIN to LPL leading to cleavage and dissociation of LPL from the cell surface, the function does not require ANGPTL3 proteolytic cleavage but seems to be mediated by the N-terminal domain, and is not inhibited by GPIHBP1 (PubMed:12909640, PubMed:16081640, PubMed:20581395). Can inhibit endothelial lipase, causing increased plasma levels of high density lipoprotein (HDL) cholesterol and phospholipids, the cleaved N-terminal domain is more efficient than the uncleaved proprotein (PubMed:17681148). Can bind to adipocytes to activate lipolysis, releasing free fatty acids and glycerol (By similarity). Suppresses LPL specifically in oxidative tissues which is required to route very low density lipoprotein (VLDL)-TG to white adipose tissue (WAT) for storage in response to food, the function may involve cooperation with circulating, liver-derived ANGPTL8 and ANGPTL4 expression in WAT (PubMed:26305978). Contributes to lower plasma levels of low density lipoprotein (LDL)-cholesterol by a mechanism that is independent of the canonical pathway implicating APOE and LDLR (PubMed:25954050). May stimulate hypothalamic LPL activity (PubMed:25338813). {ECO:0000250|UniProtKB:Q9Y5C1, ECO:0000269|PubMed:11788823, ECO:0000269|PubMed:12671033, ECO:0000269|PubMed:16081640, ECO:0000269|PubMed:17681148, ECO:0000269|PubMed:20581395, ECO:0000269|PubMed:25338813, ECO:0000269|PubMed:25954050, ECO:0000269|PubMed:26305978}., Involved in angiogenesis (PubMed:11877390). Binds to endothelial cells via integrin alpha-V/beta-3 (ITGAV:ITGB3), activates FAK, MAPK and Akt signaling pathways and induces cell adhesion and cell migration (By similarity). May increase the motility of podocytes. Secreted from podocytes, may modulate properties of glomerular endothelial cells involving integrin alpha-V/beta-3 and Akt signaling (By similarity). May induce actin filament rearrangements in podocytes implicating integrin alpha-V/beta-3 and Rac1 activation (PubMed:20633534, PubMed:24294595, PubMed:25710887). Binds to hematopoietic stem cells (HSC) and is involved in the regulation of HSC activity probably implicating down-regulation of IKZF1/IKAROS (PubMed:20959605). {ECO:0000250|UniProtKB:Q9Y5C1, ECO:0000269|PubMed:11877390, ECO:0000269|PubMed:20633534, ECO:0000269|PubMed:20959605, ECO:0000269|PubMed:24294595, ECO:0000269|PubMed:25710887}.
    Molekulargewicht
    51.7 kDa Including tag.
    UniProt
    Q9R182
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