FZD3 Protein (AA 499-666) (His tag)

Produktnummer ABIN3135346

Produktdetails

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 499-666

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses FZD3 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: GSKKTCFEWA SFFHGRRKKE IVNESRQVLQ EPDFAQSLLR DPNTPIIRKS RGTSTQGTST HASSTQLAMV DDQRSKAGSV HSKVSSYHGS LHRSRDGRYT PCSYRGMEER LPHGSMSRLT DHSRHSSSHR LNEQSRHSSI RDLSNNPMTH ITHGTSMNRV IEEDGTSA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Fzd3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Andere Bezeichnung: Fzd3 (FZD3 Produkte)

Synonyme: Fz-3 Protein, FZ-3 Protein, fz3 Protein, Xfz3 Protein, frz3 Protein, hfz3 Protein, frz-3 Protein, frizzled3 Protein, frizzled-3 Protein, FZD3 Protein, AU020229 Protein, D930050A07Rik Protein, Fz3 Protein, fz9 Protein, fzd3 Protein, zg09 Protein, fzd3l Protein, frizzled class receptor 3 Protein, frizzled class receptor 3 L homeolog Protein, frizzled class receptor 3a Protein, frizzled class receptor 3b Protein, FZD3 Protein, fzd3 Protein, fzd3.L Protein, Fzd3 Protein, fzd3a Protein, fzd3b Protein

Hintergrund: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Activation by Wnt5A stimulates PKC activity via a G-protein-dependent mechanism. Involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Plays a role in controlling early axon growth and guidance processes necessary for the formation of a subset of central and peripheral major fiber tracts. Required for the development of major fiber tracts in the central nervous system, including: the anterior commissure, the corpus callosum, the thalamocortical, corticothalamic and nigrostriatal tracts, the corticospinal tract, the fasciculus retroflexus, the mammillothalamic tract, the medial lemniscus, and ascending fiber tracts from the spinal cord to the brain. In the peripheral nervous system, controls axon growth in distinct populations of cranial and spinal motor neurons, including the facial branchimotor nerve, the hypoglossal nerve, the phrenic nerve, and motor nerves innervating dorsal limbs. Involved in the migration of cranial neural crest cells. May also be implicated in the transmission of sensory information from the trunk and limbs to the brain. Controls commissural sensory axons guidance after midline crossing along the anterior-posterior axis in the developing spinal cord in a Wnt-dependent signaling pathway. Together with FZD6, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear. Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle in a beta-catenin-dependent manner. {ECO:0000269|PubMed:12351730, ECO:0000269|PubMed:14671310, ECO:0000269|PubMed:16407530, ECO:0000269|PubMed:16495441, ECO:0000269|PubMed:21325504, ECO:0000269|PubMed:24347548, ECO:0000269|PubMed:24799694}.

Molekulargewicht: 19.9 kDa Including tag.

UniProt: Q61086

Pathways: WNT Signalweg, Tube Formation