Glucocorticoid Receptor Protein (AA 1-783) (His tag)

Produktnummer ABIN3132552

Produktdetails

Target: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-783

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses Glucocorticoid Receptor Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: MDSKESLAPP GRDEVPSSLL GRGRGSVMDL YKTLRGGATV KVSASSPSVA AASQADSKQQ RILLDFSKGS ASNAQQQQQQ QQPQPDLSKA VSLSMGLYMG ETETKVMGND LGYPQQGQLG LSSGETDFRL LEESIANLNR STSRPENPKS STPAAGCATP TEKEFPQTHS DPSSEQQNRK SQPGTNGGSV KLYTTDQSTF DILQDLEFSA GSPGKETNES PWRSDLLIDE NLLSPLAGED DPFLLEGDVN EDCKPLILPD TKPKIQDTGD TILSSPSSVA LPQVKTEKDD FIELCTPGVI KQEKLGPVYC QASFSGTNII GNKMSAISVH GVSTSGGQMY HYDMNTASLS QQQDQKPVFN VIPPIPVGSE NWNRCQGSGE DNLTSLGAMN FAGRSVFSNG YSSPGMRPDV SSPPSSSSTA TGPPPKLCLV CSDEASVCHY GVLTCGSCKV FFKRAVEGQH NYLCAGRNDC IIDKIRRKNC PACRYRKCLQ AGMNLEARKT KKKIKGIQQA TAGVSQDTSE NANKTIVPAA LPQLTPTLVS LLEVIEPEVL YAGYDSSVPD SAWRIMTTLN MLGGRQVIAA VKWAKAIPGF RNLHLDDQMT LLQYSWMFLM AFALGWRSYR QASGNLLCFA PDLIINEQRM TLPCMYDQCK HMLFISTELQ RLQVSYEEYL CMKTLLLLSS VPKEGLKSQE LFDEIRMTYI KELGKAIVKR EGNSSQNWQR FYQLTKLLDS MHDVVENLLS YCFQTFLDKS MSIEFPEMLA EIITNQIPKY SNGNIKKLLF HQK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Nr3c1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Andere Bezeichnung: Nr3c1 (NR3C1 Produkte)

Synonyme: GR Protein, gcr Protein, grl Protein, xGR Protein, gccr Protein, gr Protein, zgc:113038 Protein, NR3C1 Protein, GCL Protein, LOC100302444 Protein, Gcr Protein, Grl Protein, Grl-1 Protein, Grl1 Protein, GRL Protein, GCCR Protein, GCR Protein, NO Protein, GR-A Protein, nr3c1 Protein, nuclear receptor subfamily 3 group C member 1 Protein, nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) Protein, nuclear receptor subfamily 3, group C, member 1 Protein, glucocorticoid receptor Protein, NR3C1 Protein, nr3c1 Protein, LOC100302444 Protein, Nr3c1 Protein

Hintergrund: Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:10678832). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (By similarity). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (PubMed:15037546). {ECO:0000250|UniProtKB:P04150, ECO:0000269|PubMed:10678832, ECO:0000269|PubMed:15037546}., Isoform 1: Has transcriptional activation and repression activity (By similarity). Mediates glucocorticoid-induced apoptosis (By similarity). Promotes accurate chromosome segregation during mitosis (PubMed:25847991). May act as a tumor suppressor (PubMed:25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (PubMed:21994940). {ECO:0000250|UniProtKB:P04150, ECO:0000269|PubMed:21994940, ECO:0000269|PubMed:25847991}., Isoform 3: Acts as a dominant negative inhibitor of isoform 1 (PubMed:20660300). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (By similarity). Loses this transcription modulator function on its own (By similarity). Has no hormone-binding activity (PubMed:20660300). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (PubMed:20660300). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (By similarity). Directly regulates STAT1 expression in isoform Alpha-independent manner (By similarity). {ECO:0000250|UniProtKB:P04150, ECO:0000269|PubMed:20660300}.

Molekulargewicht: 87.0 kDa Including tag.

UniProt: P06537

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process