GUCY2C Protein (AA 455-1072) (His tag)

Produktnummer ABIN3122211

Produktdetails

Target: GUCY2C (Guanylate Cyclase 2C (Heat Stable Enterotoxin Receptor) (GUCY2C))

Protein-Typ: Recombinant

Proteineigenschaft: AA 455-1072

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses GUCY2C Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: RKYRRDHALR QKKWSHIPSE NIFPLETNET NHISLKIDDD RRRDTIQRVR QCKYDKKKVI LKDLKHSDGN FSEKQKIDLN KLLQSDYYNL TKFYGTVKLD TRIFGVVEYC ERGSLREVLN DTISYPDGTF MDWEFKISVL NDIAKGMSYL HSSKIEVHGR LKSTNCVVDS RMVVKITDFG CNSILPPKKD LWTAPEHLRQ ATISQKGDVY SFAIIAQEII LRKETFYTLS CRDHNEKIFR VENSYGKPFR PDLFLETADE KELEVYLLVK SCWEEDPEKR PDFKKIESTL AKIFGLFHDQ KNESYMDTLI RRLQLYSRNL EHLVEERTQL YKAERDRADH LNFMLLPRLV VKSLKEKGIV EPELYEEVTI YFSDIVGFTT ICKYSTPMEV VDMLNDIYKS FDQIVDHHDV YKVETIGDAY VVASGLPMRN GNRHAVDISK MALDILSFIG TFELEHLPGL PVWIRIGVHS GPCAAGVVGI KMPRYCLFGD TVNTASRMES TGLPLRIHMS SSTITILKRT DCQFLYEVRG ETYLKGRGTE TTYWLTGMKD QEYNLPSPPT VENQQRLQTE FSDMIVSALQ KRQASGKKSR RPTRVASYKK GFLEYMQLNN SDHDSTYF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Gucy2c Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: GUCY2C (Guanylate Cyclase 2C (Heat Stable Enterotoxin Receptor) (GUCY2C))

Andere Bezeichnung: Gucy2c (GUCY2C Produkte)

Synonyme: DIAR6 Protein, GUC2C Protein, MECIL Protein, MUCIL Protein, STAR Protein, AI893437 Protein, GC-C Protein, Gcc Protein, GCC Protein, gucy2c-A Protein, GUCY2C Protein, Gucy2c Protein, guanylate cyclase 2C Protein, guanylate cyclase 2c Protein, guanylate cyclase 2C L homeolog Protein, GUCY2C Protein, Gucy2c Protein, gucy2c.L Protein, gucy2c Protein

Hintergrund: Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin (By similarity). {ECO:0000250}.

Molekulargewicht: 72.8 kDa Including tag.

UniProt: Q3UWA6