Dieses GUCY2C Protein ist gelabelt mit His-Avi Tag.
Sequenz
Ser24-Gln430
Reinheit
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human GUCY2C, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-GUCY2C Antibody, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
GUCY2C
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, Crys, SDS
Crystallography grade
GUCY2C
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, Crys, SDS
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Lagerung
4 °C,-80 °C
Informationen zur Lagerung
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Guanylyl cyclase C, GC-C, STAR, GUCY2C, GUC2C, STA receptor, DIAR6, EC 4.6.1, GCC, GUC2CEC 4.6.1.2, MUCIL,Guanylyl cyclase C (GUCY2C) has canonical centrality in defense of key intestinal homeostatic mechanisms, encompassing fluid and electrolyte balance, epithelial dynamics, antitumorigenesis, and intestinal barrier function. GUCY2C may represent a new target for anti-obesity pharmacotherapy.
Molekulargewicht
48.8 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.