Connexin 43/GJA1 Protein (AA 2-382) (rho-1D4 tag)

Produktnummer ABIN3113010

Produktdetails

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-382

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses Connexin 43/GJA1 Protein ist gelabelt mit rho-1D4 tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: GDWSALGKLL DKVQAYSTAG GKVWLSVLFI FRILLLGTAV ESAWGDEQSA FRCNTQQPGC ENVCYDKSFP ISHVRFWVLQ IIFVSVPTLL YLAHVFYVMR KEEKLNKKEE ELKVAQTDGV NVDMHLKQIE IKKFKYGIEE HGKVKMRGGL LRTYIISILF KSIFEVAFLL IQWYIYGFSL SAVYTCKRDP CPHQVDCFLS RPTEKTIFII FMLVVSLVSL ALNIIELFYV FFKGVKDRVK GKSDPYHATS GALSPAKDCG SQKYAYFNGC SSPTAPLSPM SPPGYKLVTG DRNNSSCRNY NKQASEQNWA NYSAEQNRMG QAGSTISNSH AQPFDFPDDN QNSKKLAAGH ELQPLAIVDQ RPSSRASSRA SSRPRPDDLE I
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human GJA1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Andere Bezeichnung: GJA1 (GJA1 Produkte)

Synonyme: AVSD3 Protein, CX43 Protein, DFNB38 Protein, GJAL Protein, HLHS1 Protein, HSS Protein, ODDD Protein, Cx43 Protein, AU042049 Protein, AW546267 Protein, Cnx43 Protein, Cx43alpha1 Protein, Gja-1 Protein, Npm1 Protein, connexin43 Protein, cx43a1 Protein, gja1 Protein, zfCx43.3 Protein, connexin 43 Protein, gja1-A Protein, cx43 Protein, gjal Protein, oddd Protein, dfnb38 Protein, gap junction protein alpha 1 Protein, gap junction protein, alpha 1 Protein, connexin 43 Protein, gap junction protein alpha 1 L homeolog Protein, CONNEXIN 43 protein Protein, GJA1 Protein, Gja1 Protein, cx43 Protein, gja1.L Protein, gja1 Protein, CONNEXIN 43 Protein

Hintergrund: Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity). {ECO:0000250|UniProtKB:P08050, ECO:0000250|UniProtKB:P23242}.

Molekulargewicht: 44.1 kDa Including tag.

UniProt: P17302

Pathways: MAPK Signalweg, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization