NPTN Protein (AA 29-339) (His tag)

Produktnummer ABIN3098120

Produktdetails

Target: NPTN (Neuroplastin (NPTN))

Protein-Typ: Recombinant

Proteineigenschaft: AA 29-339

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses NPTN Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: MQNAGFVKSP MSETKLTGDA FELYCDVVGS PTPEIQWWYA EVNRAESFRQ LWDGARKRRV TVNTAYGSNG VSVLRITRLT LEDSGTYECR ASNDPKRNDL RQNPSITWIR AQATISVLQK PRIVTSEEVI IRDSPVLPVT LQCNLTSSSH TLTYSYWTKN GVELSATRKN ASNMEYRINK PRAEDSGEYH CVYHFVSAPK ANATIEVKAA PDITGHKRSE NKNEGQDATM YCKSVGYPHP DWIWRKKENG MPMDIVNTSG RFFIINKENY TELNIVNLQI TEDPGEYECN ATNAIGSASV VTVLRVRSHL APHHHHHH
Sequence including C-terminal His-tag.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human NPTN Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).
• Tag Location: C-terminal His Tag

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: The protein is purified from the cleared cell lysate using EDTA resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: NPTN (Neuroplastin (NPTN))

Andere Bezeichnung: NPTN (NPTN Produkte)

Synonyme: SDFR1 Protein, GP55 Protein, GP65 Protein, SDR1 Protein, np55 Protein, np65 Protein, AW554172 Protein, Sdfr1 Protein, nptn Protein, zgc:77326 Protein, neuroplastin Protein, neuroplastin L homeolog Protein, neuroplastin b Protein, NPTN Protein, Nptn Protein, nptn.L Protein, nptnb Protein

Hintergrund: Probable homophilic and heterophilic cell adhesion molecule involved in long term potentiation at hippocampal excitatory synapses through activation of p38MAPK. May also regulate neurite outgrowth by activating the FGFR1 signaling pathway. May play a role in synaptic plasticity (By similarity). {ECO:0000250}.

Molekulargewicht: 35.0 kD. Including tag.

UniProt: Q9Y639

Pathways: Regulation of long-term Neuronal Synaptic Plasticity