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Neuroplastin (NPTN) (AA 29-339) protein (His tag) Protein

NPTN Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3084717
$8,431.43
Zzgl. Versandkosten $45.00 und $24.00 Trockeneis
1 mg ABIN3084717
1 mg ABIN3084717
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 40 Werktagen
  • Target
    NPTN
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 29-339
    Spezies
    Human
    Quelle
    Insektenzellen
    • 3
    • 3
    • 2
    • 1
    • 1
    Aufreinigungstag / Konjugat
    Dieses NPTN Protein ist gelabelt mit His tag.
    Applikation
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MQNAGFVKSP MSETKLTGDA FELYCDVVGS PTPEIQWWYA EVNRAESFRQ LWDGARKRRV TVNTAYGSNG VSVLRITRLT LEDSGTYECR ASNDPKRNDL RQNPSITWIR AQATISVLQK PRIVTSEEVI IRDSPVLPVT LQCNLTSSSH TLTYSYWTKN GVELSATRKN ASNMEYRINK PRAEDSGEYH CVYHFVSAPK ANATIEVKAA PDITGHKRSE NKNEGQDATM YCKSVGYPHP DWIWRKKENG MPMDIVNTSG RFFIINKENY TELNIVNLQI TEDPGEYECN ATNAIGSASV VTVLRVRSHL APHHHHHH
    Sequence including C-terminal His-tag.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human NPTN Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).
    • Tag Location: C-terminal His Tag

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    The protein is purified from the cleared cell lysate using EDTA resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin-free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    NPTN
    Synonyme
    SDFR1, GP55, GP65, SDR1, np55, np65, AW554172, Sdfr1, nptn, zgc:77326, neuroplastin, neuroplastin L homeolog, neuroplastin b, NPTN, Nptn, nptn.L, nptnb
    Hintergrund
    Probable homophilic and heterophilic cell adhesion molecule involved in long term potentiation at hippocampal excitatory synapses through activation of p38MAPK. May also regulate neurite outgrowth by activating the FGFR1 signaling pathway. May play a role in synaptic plasticity (By similarity). {ECO:0000250}.
    Molekulargewicht
    35.0 kD. Including tag.
    UniProt
    Q9Y639
    Pathways
    Regulation of long-term Neuronal Synaptic Plasticity
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