VASP Protein (AA 2-380) (His tag)

Produktnummer ABIN3096319

Produktdetails

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-380

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses VASP Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: SETVICSSRA TVMLYDDGNK RWLPAGTGPQ AFSRVQIYHN PTANSFRVVG RKMQPDQQVV INCAIVRGVK YNQATPNFHQ WRDARQVWGL NFGSKEDAAQ FAAGMASALE ALEGGGPPPP PALPTWSVPN GPSPEEVEQQ KRQQPGPSEH IERRVSNAGG PPAPPAGGPP PPPGPPPPPG PPPPPGLPPS GVPAAAHGAG GGPPPAPPLP AAQGPGGGGA GAPGLAAAIA GAKLRKVSKQ EEASGGPTAP KAESGRSGGG GLMEEMNAML ARRRKATQVG EKTPKDESAN QEEPEARVPA QSESVRRPWE KNSTTLPRMK SSSSVTTSET QPCTPSSSDY SDLQRVKQEL LEEVKKELQK VKEEIIEAFV QELRKRGSP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human VASP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Andere Bezeichnung: VASP (VASP Produkte)

Synonyme: vasp Protein, MGC80889 Protein, wu:fb23b04 Protein, wu:fk84g05 Protein, zgc:110347 Protein, si:dkey-113g17.1 Protein, si:ch211-202c21.1 Protein, DDBDRAFT_0188463 Protein, DDBDRAFT_0229340 Protein, DDB_0188463 Protein, DDB_0229340 Protein, VASP Protein, vasodilator stimulated phosphoprotein S homeolog Protein, vasodilator stimulated phosphoprotein b Protein, protein enabled Protein, vasodilator-stimulated phosphoprotein Protein, vasodilator stimulated phosphoprotein a Protein, vasodilator stimulated phosphoprotein Protein, vasp.S Protein, vaspb Protein, LOC5573842 Protein, CpipJ_CPIJ004706 Protein, CpipJ_CPIJ004707 Protein, vasP Protein, vasp Protein, VASP Protein, vaspa Protein, Vasp Protein

Hintergrund: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. {ECO:0000269|PubMed:10087267, ECO:0000269|PubMed:10438535, ECO:0000269|PubMed:15939738, ECO:0000269|PubMed:17082196, ECO:0000269|PubMed:18559661}.

Molekulargewicht: 40.7 kDa Including tag.

UniProt: P50552

Pathways: T-Zell Rezeptor Signalweg, Regulation of Actin Filament Polymerization, Tube Formation