Topoisomerase I Protein (AA 2-765) (His tag)

Produktnummer ABIN3095963

Produktdetails

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-765

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses Topoisomerase I Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: SGDHLHNDSQ IEADFRLNDS HKHKDKHKDR EHRHKEHKKE KDREKSKHSN SEHKDSEKKH KEKEKTKHKD GSSEKHKDKH KDRDKEKRKE EKVRASGDAK IKKEKENGFS SPPQIKDEPE DDGYFVPPKE DIKPLKRPRD EDDADYKPKK IKTEDTKKEK KRKLEEEEDG KLKKPKNKDK DKKVPEPDNK KKKPKKEEEQ KWKWWEEERY PEGIKWKFLE HKGPVFAPPY EPLPENVKFY YDGKVMKLSP KAEEVATFFA KMLDHEYTTK EIFRKNFFKD WRKEMTNEEK NIITNLSKCD FTQMSQYFKA QTEARKQMSK EEKLKIKEEN EKLLKEYGFC IMDNHKERIA NFKIEPPGLF RGRGNHPKMG MLKRRIMPED IIINCSKDAK VPSPPPGHKW KEVRHDNKVT WLVSWTENIQ GSIKYIMLNP SSRIKGEKDW QKYETARRLK KCVDKIRNQY REDWKSKEMK VRQRAVALYF IDKLALRAGN EKEEGETADT VGCCSLRVEH INLHPELDGQ EYVVEFDFLG KDSIRYYNKV PVEKRVFKNL QLFMENKQPE DDLFDRLNTG ILNKHLQDLM EGLTAKVFRT YNASITLQQQ LKELTAPDEN IPAKILSYNR ANRAVAILCN HQRAPPKTFE KSMMNLQTKI DAKKEQLADA RRDLKSAKAD AKVMKDAKTK KVVESKKKAV QRLEEQLMKL EVQATDREEN KQIALGTSKL NYLDPRITVA WCKKWGVPIE KIYNKTQREK FAWAIDMADE DYEF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human TOP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Andere Bezeichnung: TOP1 (TOP1 Produkte)

Synonyme: CG6146 Protein, Dmel\\CG6146 Protein, TOP1 Protein, TopI Protein, Topo I Protein, TopoI Protein, dTopoI Protein, l(1)G0134 Protein, l(1)G0201 Protein, l(1)G0229 Protein, l(1)G0278 Protein, l(1)top1 Protein, top1 Protein, topo I Protein, topoI Protein, DNA topoisomerase 1 beta Protein, DNA topoisomerase I alpha Protein, MGO1 Protein, MGOUN 1 Protein, MTE17.1 Protein, MTE17_1 Protein, TOP1BETA Protein, TOPOISOMERASE 1 Protein, TOPI Protein, AI467334 Protein, D130064I21Rik Protein, Top-1 Protein, Ab2-086 Protein, topi Protein, Topoisomerase 1 Protein, DNA topoisomerase I Protein, DNA topoisomerase I alpha Protein, DNA topoisomerase 1 Protein, topoisomerase (DNA) I Protein, DNA topoisomerase I, gene 1 L homeolog Protein, Top1 Protein, top1 Protein, topA Protein, ZMO1193 Protein, MRAD2831_RS63095 Protein, Lferr_0016 Protein, PC1_2015 Protein, Za10_0136 Protein, Gbro_0575 Protein, Dd586_2108 Protein, Kvar_3044 Protein, Alvin_2069 Protein, Dacet_0849 Protein, Mrub_1615 Protein, Arnit_2962 Protein, Ndas_4953 Protein, Trad_2377 Protein, Acear_1653 Protein, Mahau_0844 Protein, Mesop_4109 Protein, Thein_1586 Protein, Theth_0241 Protein, TOP1ALPHA Protein, top-1 Protein, TOP1 Protein, top1.1.L Protein

Substanzklasse: Viral Protein

Hintergrund: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter. {ECO:0000250|UniProtKB:Q13472, ECO:0000269|PubMed:14594810, ECO:0000269|PubMed:16033260, ECO:0000269|PubMed:19168442, ECO:0000269|PubMed:22904072, ECO:0000269|PubMed:2833744}.

Molekulargewicht: 91.5 kDa Including tag.

UniProt: P11387

Pathways: Caspase Kaskade in der Apoptose, Stem Cell Maintenance