Topoisomerase I Protein (His tag)

Produktnummer ABIN6126489

Dieses Recombinant Topoisomerase I-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Topoisomerase I Protein (His tag) (ABIN6126489)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB), SDS-PAGE (SDS)

Reinheit: > 80 %

Produktdetails

Aufreinigungstag / Konjugat: Dieses Topoisomerase I Protein ist gelabelt mit His tag.

Verwendungszweck: Mouse Topoisomerase I (TOP1) Protein

Sequenz: MSGDHLHNDS QIEADFRLND SHKHKDKHKD REHRHKEHKK DKDKDREKSK HSNSEHKDSE KKHKEKEKTK HKDGSSEKHK DKHKDRDKER RKEEKIRAAG DAKIKKEKEN GFSSPPRIKD EPEDDGYFAP PKEDIKPLKR LRDEDDADYK PKKIKTEDIK KEKKRKSEEE EDGKLKKPKN KDKDKKVAEP DNKKKKPKKE EEQKWKWWEE ERYPEGIKWK FLEHKGPVFA PPYEPLPESV KFYYDGKVMK LS

Biological Activity Comment: Not tested

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

Buffer: Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 5 % Trehalose.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.

Antigendetails

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Andere Bezeichnung: Topoisomerase I

Substanzklasse: Viral Protein

Molekulargewicht: Calculated MW: 34.1 kDa
Observed MW (SDS-PAGE): 38 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.

Gen-ID

21969

UniProt: Q04750

Pathways: Caspase Kaskade in der Apoptose, Stem Cell Maintenance