NLRP10 Protein (AA 1-655) (Strep Tag)

Produktnummer ABIN3094044

Produktdetails

Target: NLRP10 (NLR Family, Pyrin Domain Containing 10 (NLRP10))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-655

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses NLRP10 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequenz: MAMAKARKPR EALLWALSDL EENDFKKLKF YLRDMTLSEG QPPLARGELE GLIPVDLAEL LISKYGEKEA VKVVLKGLKV MNLLELVDQL SHICLHDYRE VYREHVRCLE EWQEAGVNGR YNQVLLVAKP SSESPESLAC PFPEQELESV TVEALFDSGE KPSLAPSLVV LQGSAGTGKT TLARKMVLDW ATGTLYPGRF DYVFYVSCKE VVLLLESKLE QLLFWCCGDN QAPVTEILRQ PERLLFILDG FDELQRPFEE KLKKRGLSPK ESLLHLLIRR HTLPTCSLLI TTRPLALRNL EPLLKQARHV HILGFSEEER ARYFSSYFTD EKQADRAFDI VQKNDILYKA CQVPGICWVV CSWLQGQMER GKVVLETPRN STDIFMAYVS TFLPPDDDGG CSELSRHRVL RSLCSLAAEG IQHQRFLFEE AELRKHNLDG PRLAAFLSSN DYQLGLAIKK FYSFRHISFQ DFFHAMSYLV KEDQSRLGKE SRREVQRLLE VKEQEGNDEM TLTMQFLLDI SKKDSFSNLE LKFCFRISPC LAQDLKHFKE QMESMKHNRT WDLEFSLYEA KIKNLVKGIQ MNNVSFKIKH SNEKKSQSQN LFSVKSSLSH GPKEEQKCPS VHGQKEGKDN IAGTQKEAST GKGRGTEETP KNTYI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: NLRP10 (NLR Family, Pyrin Domain Containing 10 (NLRP10))

Andere Bezeichnung: NLRP10 (NLRP10 Produkte)

Synonyme: CLR11.1 Protein, NALP10 Protein, NOD8 Protein, PAN5 Protein, PYNOD Protein, 6430548I20Rik Protein, Nalp10 Protein, Napl10 Protein, Pynod Protein, NLR family pyrin domain containing 10 Protein, NLR family, pyrin domain containing 10 Protein, NLRP10 Protein, Nlrp10 Protein

Hintergrund: NACHT, LRR and PYD domains-containing protein 10 (Nucleotide-binding oligomerization domain protein 8),FUNCTION: Inhibits autoprocessing of CASP1, CASP1-dependent IL1B secretion, PYCARD aggregation and PYCARD-mediated apoptosis but not apoptosis induced by FAS or BID (PubMed:15096476). Displays anti-inflammatory activity (PubMed:20393137). Required for immunity against C.albicans infection (By similarity). Involved in the innate immune response by contributing to pro-inflammatory cytokine release in response to invasive bacterial infection (PubMed:22672233). Contributes to T-cell-mediated inflammatory responses in the skin (By similarity). Plays a role in protection against periodontitis through its involvement in induction of IL1A via ERK activation in oral epithelial cells infected with periodontal pathogens (PubMed:28766990). Exhibits both ATPase and GTPase activities (PubMed:23861819). {ECO:0000250|UniProtKB:Q8CCN1, ECO:0000269|PubMed:15096476, ECO:0000269|PubMed:20393137, ECO:0000269|PubMed:22672233, ECO:0000269|PubMed:23861819}.

Molekulargewicht: 75.0 kDa

UniProt: Q86W26

Pathways: Inflammasome