DYNC1I2 Protein (AA 2-638) (His tag)

Produktnummer ABIN3092043

Produktdetails

Target: DYNC1I2 (Dynein, Cytoplasmic 1, Intermediate Chain 2 (DYNC1I2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-638

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses DYNC1I2 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: SDKSELKAEL ERKKQRLAQI REEKKRKEEE RKKKETDQKK EAVAPVQEES DLEKKRREAE ALLQSMGLTP ESPIVFSEYW VPPPMSPSSK SVSTPSEAGS QDSGDGAVGS RTLHWDTDPS VLQLHSDSDL GRGPIKLGMA KITQVDFPPR EIVTYTKETQ TPVMAQPKED EEEDDDVVAP KPPIEPEEEK TLKKDEENDS KAPPHELTEE EKQQILHSEE FLSFFDHSTR IVERALSEQI NIFFDYSGRD LEDKEGEIQA GAKLSLNRQF FDERWSKHRV VSCLDWSSQY PELLVASYNN NEDAPHEPDG VALVWNMKYK KTTPEYVFHC QSAVMSATFA KFHPNLVVGG TYSGQIVLWD NRSNKRTPVQ RTPLSAAAHT HPVYCVNVVG TQNAHNLISI STDGKICSWS LDMLSHPQDS MELVHKQSKA VAVTSMSFPV GDVNNFVVGS EEGSVYTACR HGSKAGISEM FEGHQGPITG IHCHAAVGAV DFSHLFVTSS FDWTVKLWTT KNNKPLYSFE DNADYVYDVM WSPTHPALFA CVDGMGRLDL WNLNNDTEVP TASISVEGNP ALNRVRWTHS GREIAVGDSE GQIVIYDVGE QIAVPRNDEW ARFGRTLAEI NANRADAEEE AATRIPA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human DYNC1I2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: DYNC1I2 (Dynein, Cytoplasmic 1, Intermediate Chain 2 (DYNC1I2))

Andere Bezeichnung: DYNC1I2 (DYNC1I2 Produkte)

Synonyme: dnci2 Protein, dncic1 Protein, ic2 Protein, dync1i2 Protein, im:7147021 Protein, zgc:153318 Protein, 3110079H08Rik Protein, AW554389 Protein, Dncic2 Protein, Dnci2 Protein, DNCI2 Protein, IC2 Protein, dynein, cytoplasmic 1, intermediate chain 2 Protein, dynein, cytoplasmic 1, intermediate chain 2 S homeolog Protein, dynein cytoplasmic 1 intermediate chain 2 Protein, dynein, cytoplasmic 1, intermediate chain 2a Protein, dync1i2 Protein, dync1i2.S Protein, DYNC1I2 Protein, dync1i2a Protein, Dync1i2 Protein

Hintergrund: Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes.

Molekulargewicht: 72.3 kDa Including tag.

UniProt: Q13409

Pathways: M Phase