B3GAT3 Protein (AA 29-335) (His tag)

Produktnummer ABIN3089651

Produktdetails

Target: B3GAT3 (beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 29-335

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses B3GAT3 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: QPCDCLPPLR AAAEQLRQKD LRISQLQAEL RRPPPAPAQP PEPEALPTIY VVTPTYARLV QKAELVRLSQ TLSLVPRLHW LLVEDAEGPT PLVSGLLAAS GLLFTHLVVL TPKAQRLREG EPGWVHPRGV EQRNKALDWL RGRGGAVGGE KDPPPPGTQG VVYFADDDNT YSRELFEEMR WTRGVSVWPV GLVGGLRFEG PQVQDGRVVG FHTAWEPSRP FPVDMAGFAV ALPLLLDKPN AQFDSTAPRG HLESSLLSHL VDPKDLEPRA ANCTRVLVWH TRTEKPKMKQ EEQLQRQGRG SDPAIEV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human B3GAT3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: B3GAT3 (beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3))

Andere Bezeichnung: B3GAT3 (B3GAT3 Produkte)

Synonyme: GLCATI Protein, 2810405M13Rik Protein, GlcUAT-I Protein, Glcat-i Protein, beta-1,3-glucuronyltransferase 3 Protein, beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) Protein, B3GAT3 Protein, B3gat3 Protein

Hintergrund: Glycosaminoglycans biosynthesis (PubMed:25893793). Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation (PubMed:24425863). {ECO:0000269|PubMed:24425863, ECO:0000269|PubMed:25893793}.

Molekulargewicht: 35.0 kDa Including tag.

UniProt: O94766

Pathways: Glycosaminoglycan Metabolic Process