CEP290 Protein (AA 1-164) (Strep Tag)

Produktnummer ABIN3082903

Produktdetails

Target: CEP290 (Centrosomal Protein 290kDa (CEP290))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-164

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses CEP290 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MSSCPVHDCP SWDPERLEPV ETGSRGALRL RGGAPGSAAG FRASIWGPAG YPSPVGLGHP ASLPRPAYSP RCPEPDARHG WGSGSNAGYR GPDRAGRTPC PAQDREGRSS SPVPPPRLKA MTSQARKQNG GALIDTVDWT REAPDSDPVM SMQKTQKPQT TVGQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CEP290 (Centrosomal Protein 290kDa (CEP290))

Andere Bezeichnung: LCA10 (CEP290 Produkte)

Synonyme: ct87 Protein, mks4 Protein, rd16 Protein, bbs14 Protein, jbts5 Protein, jbts6 Protein, lca10 Protein, nphp6 Protein, slsn6 Protein, sh11ag Protein, MGC81471 Protein, 3h11ag Protein, RGD1311640 Protein, BC004690 Protein, Nphp6 Protein, b2b1454Clo Protein, b2b1752Clo Protein, im:7145703 Protein, im:7147820 Protein, 3H11Ag Protein, BBS14 Protein, CT87 Protein, JBTS5 Protein, LCA10 Protein, MKS4 Protein, NPHP6 Protein, POC3 Protein, SLSN6 Protein, centrosomal protein 290 Protein, centrosomal protein 290 L homeolog Protein, centrosomal protein 290kDa Protein, CEP290 Protein, cep290.L Protein, cep290 Protein, Cep290 Protein

Hintergrund: Putative lung carcinoma-associated protein 10

Molekulargewicht: 17.3 kDa

UniProt: Q71F78

Pathways: cAMP Metabolic Process, M Phase