FH Protein (AA 45-510) (His tag)

Produktnummer ABIN3080328

Produktdetails

Target: FH (Fumarate Hydratase (FH))

Protein-Typ: Recombinant

Proteineigenschaft: AA 45-510

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses FH Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: ASQNSFRIEY DTFGELKVPN DKYYGAQTVR STMNFKIGGV TERMPTPVIK AFGILKRAAA EVNQDYGLDP KIANAIMKAA DEVAEGKLND HFPLVVWQTG SGTQTNMNVN EVISNRAIEM LGGELGSKIP VHPNDHVNKS QSSNDTFPTA MHIAAAIEVH EVLLPGLQKL HDALDAKSKE FAQIIKIGRT HTQDAVPLTL GQEFSGYVQQ VKYAMTRIKA AMPRIYELAA GGTAVGTGLN TRIGFAEKVA AKVAALTGLP FVTAPNKFEA LAAHDALVEL SGAMNTTACS LMKIANDIRF LGSGPRSGLG ELILPENEPG SSIMPGKVNP TQCEAMTMVA AQVMGNHVAV TVGGSNGHFE LNVFKPMMIK NVLHSARLLG DASVSFTENC VVGIQANTER INKLMNESLM LVTALNPHIG YDKAAKIAKT AHKNGSTLKE TAIELGYLTA EQFDEWVKPK DMLGPK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human FH Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: FH (Fumarate Hydratase (FH))

Andere Bezeichnung: FH (FH Produkte)

Synonyme: FH Protein, DDBDRAFT_0205237 Protein, DDBDRAFT_0231397 Protein, DDBDRAFT_0231400 Protein, DDB_0205237 Protein, DDB_0231397 Protein, DDB_0231400 Protein, HLRCC Protein, LRCC Protein, MCL Protein, MCUL1 Protein, Fh1 Protein, im:7152785 Protein, ns:zf-e152 Protein, zf-e152 Protein, zgc:66253 Protein, zgc:77498 Protein, Fh Protein, Fh-1 Protein, fumarate hydratase Protein, fumarate hydratase 1 Protein, FH Protein, fumH Protein, Fh Protein, fh Protein, Fh1 Protein

Hintergrund: Also acts as a tumor suppressor.

Molekulargewicht: 51.0 kDa Including tag.

UniProt: P07954