Glycoprotein / GP (Virus) (AA 33-324) protein (His tag)

Produktnummer ABIN2870777

Produktdetails

Target: Glycoprotein / GP (Virus)

Protein-Typ: Recombinant

Proteineigenschaft: AA 33-324

Spezies: Ebola Virus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: His tag

Sequenz: AA 33-324

Produktmerkmale: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 34.3 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE).

Reinheit: >95 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Antigendetails

Target: Glycoprotein / GP (Virus)

Substanzklasse: Viral Protein

Hintergrund: EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160- kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.

Molekulargewicht: 34.3 kDa

UniProt: B8XCN1