NMNAT3 Protein (AA 1-252) (His tag)

Produktnummer ABIN2745774

Produktdetails

Target: NMNAT3 (Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-252

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses NMNAT3 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Kreuzreaktivität: Human

Produktmerkmale: Human full-length NMNAT3 (aa 1-252) is fused at the N-terminus to a His-tag.

Reinheit: >95 % (SDS-PAGE)

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

>2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1μmol of NAD+ per min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: In 50 mM TRIS-HCl, pH 8.0, containing 300 mM sodium chloride, 1 mM DTT and 10 % glycerol.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Working aliquots are stable for up to 3 months when stored at -20°C.

Antigendetails

Target: NMNAT3 (Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3))

Andere Bezeichnung: NMNAT3 (NMNAT3 Produkte)

Synonyme: PNAT-3 Protein, PNAT3 Protein, 4933408N02Rik Protein, nicotinamide nucleotide adenylyltransferase 3 Protein, si:ch73-170l17.1 Protein, Nmnat3 Protein, NMNAT3 Protein, si:ch73-170l17.1 Protein

Hintergrund: Mitochondrial NMNAT isoform. Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form of nicotinic acid mononucleotide (NAMN) as substrate with the same efficiency. Can use tiazofurin monophosphate as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity, mitochondrial NMNAT isoform can use NAD(+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NAADP+. Protects against axonal degeneration following injury.

Molekulargewicht: ~27kDa (monomer)

UniProt: Q96T66