TIGAR Protein (TAT tag)
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- Target Alle TIGAR Proteine anzeigen
- TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses TIGAR Protein ist gelabelt mit TAT tag.
- Applikation
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- Spezifität
- Optimal preservation of protein structure, post-translational modifications and functions.
- Produktmerkmale
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- Recombinant human TIGAR protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Reinheit
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin-Niveau
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Pretreatment with TIGAR-TAT for 4 hrs, using a concentration range 0.1-5.0 ug/mL, protects U2OS cells from apoptosis induced by hydrogen peroxide.
- Top Product
- Discover our top product TIGAR Protein
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TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 2-270) protein (His tag)
TIGAR Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-270) protein (GST tag)TIGAR Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AA, AP
TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-270) protein (Strep Tag)Crystallography grade TIGAR Spezies: Human Wirt: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-269) protein (Strep Tag)Crystallography grade TIGAR Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
TP53 induced glycolysis regulatory phosphatase (TIGAR) protein (Myc-DYKDDDDK Tag)TIGAR Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Applikationshinweise
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handhabung
- Resuspend the protein in the desired concentration in proper buffer
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))
- Andere Bezeichnung
- Tigar (TIGAR Produkte)
- Synonyme
- AA793651 Protein, AI595337 Protein, C79710 Protein, C85509 Protein, Tigar Protein, Trp53 induced glycolysis repulatory phosphatase Protein, Tigar Protein
- Hintergrund
- This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region.
- Molekulargewicht
- 31.6 kDa
- NCBI Accession
- NP_065108
- Pathways
- Warburg Effekt
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