PTTG1 Protein (Myc-DYKDDDDK Tag)

Produktnummer ABIN2731599

Produktdetails

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses PTTG1 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human Securin / PTTG1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Andere Bezeichnung: Securin,pttg1 (PTTG1 Produkte)

Synonyme: EAP1 Protein, HPTTG Protein, PTTG Protein, TUTR1 Protein, PTTG1 Protein, securin Protein, AW555095 Protein, C87862 Protein, Pttg Protein, Pttg3 Protein, Securin Protein, Protein Cut2 Protein, pituitary tumor-transforming 1 Protein, pituitary tumor-transforming gene 1 Protein, securin, sister chromatid separation inhibitor Protein, PTTG1 Protein, Pttg1 Protein, cut2 Protein

Hintergrund: The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene.

Molekulargewicht: 21.8 kDa

NCBI Accession: NP_004210