PPP1R12A Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Produktnummer ABIN2729468

Produktdetails

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Protein-Typ: Recombinant

Proteineigenschaft: Transcript Variant 1

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses PPP1R12A Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human PPP1R12A / MYPT1 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Andere Bezeichnung: Ppp1r12a,mypt1 (PPP1R12A Produkte)

Synonyme: 1200015F06Rik Protein, 5730577I22Rik Protein, AA792106 Protein, AV099298 Protein, D10Ertd625e Protein, Mypt1 Protein, M130 Protein, MBS Protein, MYPT1 Protein, M110 Protein, MBSP Protein, protein phosphatase 1, regulatory (inhibitor) subunit 12A Protein, protein phosphatase 1 regulatory subunit 12A Protein, protein phosphatase 1, regulatory subunit 12A Protein, Ppp1r12a Protein, PPP1R12A Protein

Hintergrund: Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 115.1 kDa

NCBI Accession: NP_002471

Pathways: M Phase