CRYAA Protein (Myc-DYKDDDDK Tag)

Produktnummer ABIN2714620

Produktdetails

Target: CRYAA (Crystallin, alpha A (CRYAA))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CRYAA Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human Alpha-crystallin A chain / CRYA1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: CRYAA (Crystallin, alpha A (CRYAA))

Andere Bezeichnung: alpha-Crystallin A Chain (CRYAA Produkte)

Synonyme: CRYA1 Protein, CTRCT9 Protein, HSPB4 Protein, AI323437 Protein, Acry-1 Protein, Crya-1 Protein, Crya1 Protein, DAcry-1 Protein, lop18 Protein, alpha-a-crystallin Protein, cryaa-A Protein, CRYAA Protein, wu:fc06h04 Protein, zgc:92036 Protein, HspB4 Protein, crystallin alpha A Protein, crystallin, alpha A Protein, alphaA-crystallin Protein, alpha-crystallin A chain-like Protein, crystallin alpha A S homeolog Protein, alpha-crystallin A chain Protein, CRYAA Protein, Cryaa Protein, CpipJ_CPIJ005646 Protein, cryaa Protein, LOC100384978 Protein, cryaa.S Protein, LOC458589 Protein, LOC102724652 Protein

Hintergrund: Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).

Molekulargewicht: 19.7 kDa

NCBI Accession: NP_000385

Pathways: M Phase