alpha Actinin 4 Protein (Myc-DYKDDDDK Tag)

Produktnummer ABIN2714614

Produktdetails

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses alpha Actinin 4 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human Alpha-actinin-4 / ACTN4 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Referenzen

Wang, Li, Wang, Tan, Jia, Yang, Li, Shang, Xu, Yang, Shoyama, Cai: "Alpha-Actinin-4 is a Possible Target Protein for Aristolochic Acid I in Human Kidney Cells In Vitro." in: The American journal of Chinese medicine, Vol. 44, Issue 2, pp. 291-304, (2016) (PubMed).

Antigendetails

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Andere Bezeichnung: alpha-Actinin-4,actn4 (ACTN4 Produkte)

Synonyme: fsgs Protein, fsgs1 Protein, C77391 Protein, ACTININ-4 Protein, FSGS Protein, FSGS1 Protein, wu:fb53f05 Protein, zgc:63508 Protein, actinin alpha 4 Protein, actinin, alpha 4 Protein, actinin alpha 4 S homeolog Protein, actn4 Protein, ACTN4 Protein, Actn4 Protein, actn4.S Protein

Hintergrund: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Molekulargewicht: 104.7 kDa

NCBI Accession: NP_004915

Pathways: Proton Transport