alpha Actinin 4 Protein (AA 1-912) (Strep Tag)

Produktnummer ABIN3134525

Dieses Recombinant alpha Actinin 4-Protein wird in Cell-free protein synthesis (CFPS) produziert.

Kurzübersicht für alpha Actinin 4 Protein (AA 1-912) (Strep Tag) (ABIN3134525)

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Cell-free protein synthesis (CFPS)

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Reinheit: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-912

Aufreinigungstag / Konjugat: Dieses alpha Actinin 4 Protein ist gelabelt mit Strep Tag.

Sequenz: MVDYHAANQA YQYGPNSGGG NGAGGGGSMG DYMAQEDDWD RDLLLDPAWE KQQRKTFTAW CNSHLRKAGT QIENIDEDFR DGLKLMLLLE VISGERLPKP ERGKMRVHKI NNVNKALDFI ASKGVKLVSI GAEEIVDGNA KMTLGMIWTI ILRFAIQDIS VEETSAKEGL LLWCQRKTAP YKNVNVQNFH ISWKDGLAFN ALIHRHRPEL IEYDKLRKDD PVTNLNNAFE VAEKYLDIPK MLDAEDIVNT ARPDEKAIMT YVSSFYHAFS GAQKAETAAN RICKVLAVNQ ENEHLMEDYE RLASDLLEWI RRTIPWLEDR VPQKTIQEMQ QKLEDFRDYR RVHKPPKVQE KCQLEINFNT LQTKLRLSNR PAFMPSEGRM VSDINNGWQH LEQAEKGYEE WLLNEIRRLE RLDHLAEKFR QKASIHEAWT DGKEAMLKQR DYETATLSDI KALIRKHEAF ESDLAAHQDR VEQIAAIAQE LNELDYYDSH NVNTRCQKIC DQWDNLGSLT HSRREALEKT EKQLETIDQL HLEYAKRAAP FNNWMESAME DLQDMFIVHT IEEIEGLISA HDQFKSTLPD ADREREAILA IHKEAQRIAE SNHIKLSGSN PYTTVTPQII NSKWEKVQQL VPKRDHALLE EQSKQQSNEH LRRQFASQAN MVGPWIQTKM EEIGRISIEM NGTLEDQLSH LKQYERSIVD YKPSLDLLEQ QHQLIQEALI FDNKHTNYTM EHIRVGWEQL LTTIARTINE VENQILTRDA KGISQEQMQE FRASFNHFDK DHGGALGPEE FKACLISLGY DVENDRQGDA EFNRIMSVVD PNHSGLVTFQ AFIDFMSRET TDTDTADQVI ASFKVLAGDK NFITAEELRR ELPPDQAEYC IARMAPYQGP DAAPGALDYK SFSTALYGES DL
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Andere Bezeichnung: Actn4

Hintergrund: Alpha-actinin-4 (Non-muscle alpha-actinin 4),FUNCTION: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (By similarity). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (PubMed:18332111). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (By similarity). {ECO:0000250|UniProtKB:O43707, ECO:0000269|PubMed:18332111}.

Molekulargewicht: 105.0 kDa

UniProt: P57780

Pathways: Proton Transport