KDM5C Protein (DYKDDDDK Tag)

Produktnummer ABIN2669682

Produktdetails

Target: KDM5C (Lysine (K)-Specific Demethylase 5C (KDM5C))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus

Aufreinigungstag / Konjugat: Dieses KDM5C Protein ist gelabelt mit DYKDDDDK Tag.

Applikation: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Produktmerkmale: Recombinant JARID1C / KDM5C (accession number NP_004178.2) was expressed in Sf9 and contains an N-terminal FLAG tag with a molecular weight of 180 kDa.

Anwendungsinformationen

Applikationshinweise: Recombinant JARID1C / KDM5C is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me3 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 100 nM Recombinant JARID1C / KDM5C protein, and 3.3 μM H3K4me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Antigendetails

Target: KDM5C (Lysine (K)-Specific Demethylase 5C (KDM5C))

Andere Bezeichnung: JARID1C / KDM5C (KDM5C Produkte)

Synonyme: DXS1272E Protein, JARID1C Protein, MRXJ Protein, MRXSCJ Protein, MRXSJ Protein, SMCX Protein, XE169 Protein, Jarid1c Protein, Smcx Protein, mKIAA0234 Protein, histone demethylase JARID1C Protein, lysine-specific demethylase 5C Protein, lysine demethylase 5C Protein, lysine (K)-specific demethylase 5C Protein, MGYG_04221 Protein, LOC100075501 Protein, KDM5C Protein, kdm5c Protein, Kdm5c Protein

Hintergrund: Lysine (K)-specific demethylase 5C (KDM5C), also known as Jumonji, AT rich interactive domain 1C (JARID1C), is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. It is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5C participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and CoREST pAb at neuron-restrictive silencer elements. Mutations in KDM5C have been associated with X-linked mental retardation.

Molekulargewicht: 180 kDa

Pathways: Warburg Effekt