FGF7 Protein (AA 32-194)

Produktnummer ABIN2667546

Produktdetails

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 32-194

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Biochemical Assay (BCA)

Reinheit: >95 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Endotoxin-Niveau:

Less than 0.1 ng per μg of protein.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by KGF-responsive BaF3 cells is ≤ 10 ng/ml corresponding to a specific activity of ≥ 1 x 105 units/mg.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For maximum results, quick spin vial prior to opening. Reconstitute in 20 mM Tris, pH 8.0 to a concentration of ≤1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored at -20°C or -70°C.

Antigendetails

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Andere Bezeichnung: KGF (FGF7 Produkte)

Synonyme: HBGF-7 Protein, KGF Protein, Kgf Protein, kgf Protein, FGF-7 Protein, fgf7 Protein, fibroblast growth factor 7 Protein, fibroblast growth factor 7 L homeolog Protein, FGF7 Protein, Fgf7 Protein, fgf7 Protein, fgf7.L Protein

Hintergrund: KGF is a heparin binding growth factor secreted by cells of mesenchymal origin. KGF acts in a paracrine manner by binding exclusively to a splicing variant of FGF receptor 2, FGFR2-IIIB. FGFR-IIIB is predominatly expressed by epithelial cells. This suggests that KGF may play an important role in mesenchymal-epithelial communication. KGF can stimulate proliferation, migration, and differentiation of epithelial cells in a variety of tissues. In skin, KGF can be strongly upregulated in dermal fibroblasts after skin injury. KGF can induce keratinocyte proliferation and migration. KGF can also protect epithelial cells from reactive oxygen species. In liver, KGF is required for adult liver regeneration. In brain, KGF is important for inhibitory synapse formation in the hippocampus. KGF-deficient mice are prone to epileptic seizures induced by chemical kindling in adults. KGF signaling is also important for hair follicle development. Injection of recombinant KGF into adult athymic nude mice can induce dose-dependent hair growth. In lung, KGF can protect animals from oxidant-induced injury. Taken together, KGF is a potential target for epithelial tissue repair.

Molekulargewicht: The 163 amino acid recombinant protein has a predicted molecular mass of approximately 18.9 kDa. The predicted N-terminal amino acid is Cys.

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung