FGF7 Protein

Produktnummer ABIN2452198

Produktdetails

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB)

Produktmerkmale: The human FGF-7 lacking the signal sequence (1-31 aa) was expressed in E. coli and purified by the chromatographic procedures. This product is an intact enzyme without tag with 19 kDa size.

Reinheit: > 95 % as determined by SDS-PAGE (CBB staining)

Sterilität: Sterile filtered

Anwendungsinformationen

Applikationshinweise: 1. Mitogen for epithelial cells
2. Western blot control for anti-FGF-7 antibodies
3. Acceleration of wound healing is implied.
4. Acceleration of hair development is implied.

Kommentare:

Activity: The ED50 as determined by a cell proliferation assay using MTS assay kit (CellTiter 96,Promega) with human keratinocyte JCRB141 cells was < 10 ng/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: PBS (10 mM Na-phosphate, 150 mM NaCl) pH 7.2, 50 % glycerol

Handhabung: Avoid repeated freeze-thaw.
Quick freezing with liquid nitrogen and rapid thawing on water is desirable when necessary.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store at -20 C (For long term storage: -80 C).

Referenzen

Aaronson, Bottaro, Miki, Ron, Finch, Fleming, Ahn, Taylor, Rubin: "Keratinocyte growth factor. A fibroblast growth factor family member with unusual target cell specificity." in: Annals of the New York Academy of Sciences, Vol. 638, pp. 62-77, (1992) (PubMed).

Rubin, Osada, Finch, Taylor, Rudikoff, Aaronson: "Purification and characterization of a newly identified growth factor specific for epithelial cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 3, pp. 802-6, (1989) (PubMed).

Antigendetails

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Andere Bezeichnung: Keratinocyte Growth Factor (KGF/FGF7) (FGF7 Produkte)

Synonyme: HBGF-7 Protein, KGF Protein, Kgf Protein, kgf Protein, FGF-7 Protein, fgf7 Protein, fibroblast growth factor 7 Protein, fibroblast growth factor 7 L homeolog Protein, FGF7 Protein, Fgf7 Protein, fgf7 Protein, fgf7.L Protein

Hintergrund: Keratinocyte Growth Factor (KGF), also known as Fibroblast Growth Factor 7 (FGF-7), is a member of fibroblast growth factor (FGF) family. Although FGF-7 has heparin binding activity similar to FGF-1, its mitogenic activity is predominantly exhibited in keratinocytes. It is not effective to fibroblasts and endothelial cells.

Gen-ID: 2252

UniProt: P21781

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung