FASL Protein (AA 134-281) (His tag)

Produktnummer ABIN2181073

Produktdetails

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 134-281

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses FASL Protein ist gelabelt mit His tag.

Sequenz: AA 134-281

Produktmerkmale: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 17.7 kDa. The protein migrates as 25-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Reinheit: >90 % as determined by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

Referenzen

Li, Xiong, Yang, Zhou, Wu, Luo, Zhou, Liu, Li, Song, Zheng: "Endothelial Cell Apoptosis Induces TGF-β Signaling-Dependent Host Endothelial-Mesenchymal Transition to Promote Transplant Arteriosclerosis." in: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, Vol. 15, Issue 12, pp. 3095-111, (2016) (PubMed).

Antigendetails

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Andere Bezeichnung: Fas Ligand (FASL Produkte)

Synonyme: ALPS1B Protein, APT1LG1 Protein, APTL Protein, CD178 Protein, CD95-L Protein, CD95L Protein, FASL Protein, TNFSF6 Protein, fasl Protein, Fas-L Protein, Faslg Protein, Tnfsf6 Protein, gld Protein, Apt1Lg1 Protein, Fasl Protein, FASLG Protein, zgc:162027 Protein, Fas ligand Protein, Fas ligand L homeolog Protein, Fas ligand (TNF superfamily, member 6) Protein, FASLG Protein, faslg.L Protein, Fasl Protein, Faslg Protein, faslg Protein

Hintergrund: Fas ligand is also known as FasL, CD178, CD95L, or TNFSF6, is a homotrimeric type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Mature human Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 80 aa cytoplasmic domain. Within the ECD, human Fas Ligand shares 81 % and 78 % aa sequence identity with mouse and rat Fas Ligand, respectively. Apoptosis triggered by Fas-Fas ligand binding plays a fundamental role in the regulation of the immune system. Its functions include:T-cell homeostasis, cytotoxic T-cell activity, immune privilege, maternal tolerance, tumor counterattack. Defective Fas-mediated apoptosis may lead to oncogenesis as well as drug resistance in existing tumors. Germline mutation of Fas is associated with autoimmune lymphoproliferative syndrome (ALPS), a childhood disorder of apoptosis.

Molekulargewicht: 18.8 kDa

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity