UBE2C Protein (His tag)

Produktnummer ABIN2018432

Produktdetails

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses UBE2C Protein ist gelabelt mit His tag.

Sequenz: MHHHHHHAMG IRMASQNRDP AATSVAAARK GAEPSGGAAR GPVGKRLQQE LMTLMMSGDK GISAFPESDN LFKWVGTIHG AAGTVYEDLR YKLSLEFPSG YPYNAPTVKF LTPCYHPNVD TQGNICLDIL KEKWSALYDV RTILLSIQSL LGEPNIDSPL NTHAAELWKN PTAFKKYLQE TYSKQVTSQE P

Reinheit: > 95 % by SDS-PAGE and HPLC analyses.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1 EU/μg of rHuUBE2C, His as determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Buffer: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.

Antigendetails

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Andere Bezeichnung: UBE2C/UBCH10 (UBE2C Produkte)

Synonyme: LOC458287 Protein, UBCH10 Protein, dJ447F3.2 Protein, 1110015A16Rik Protein, D2Ertd695e Protein, im:7137135 Protein, zgc:123190 Protein, ubiquitin conjugating enzyme E2 C Protein, ubiquitin-conjugating enzyme E2C Protein, ubiquitin conjugating enzyme E2 C L homeolog Protein, ubiquitin conjugating enzyme E2 C S homeolog Protein, UBE2C Protein, NAEGRDRAFT_82946 Protein, ube2c.L Protein, Ube2c Protein, ube2c.S Protein, ube2c Protein

Hintergrund: Human Ubquitin Conjugating Enzyme E2 C (UBE2C)/UBCH10 is an essential mediator of mitotic destruction events and cell cycle progression. It catalyzes the destruction of cyclins A and B in conjunction with the anaphase-promoting complex, and therefore, plays an important role in the control of the cell exit from mitosis This activity is essential at then end of mitosis for the inactivation of their partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle. In addition, UBCH10 bears homology to yeast PAS2, a gene that is essential for biogenesis of peroxisomes. UBCH10 is useful for in vitro ubiquitinylation reactions.
Synonyms: Ubiquitin-conjugating enzyme E2 C, EC 6.3.2.19, Ubiquitin-protein ligase C, Ubiquitin carrier protein C, Ubc10, UBCH10, dJ447F3.2.

Molekulargewicht: 21.1 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids (a.a.) of human UBE2C/UBCH10 and 12 a.a. vector sequence including 6 x His tag at N-terminus.

Pathways: Ubiquitin Proteasome Pathway