PTPN13 Protein

Produktnummer ABIN2018353

Produktdetails

Target: PTPN13 (Protein tyrosine Phosphatase, Non-Receptor Type 13 (APO-1/CD95 (Fas)-Associated Phosphatase) (PTPN13))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Sequenz: RLSSKSVNAQ VTDINSKGLE LRKTVTTVET QNLEGLHHDG QFCHKPCPPG ERKARDCTVN GDEPDCVPCQ EGKEYTDKAH FSSKCRRCRL CDEGHGLEVE INCTRTQNTK CRCKPNFFCN STVCEHCDPC TKCEHGIIKE CTLTSNTKCK EEGSRSN

Produktmerkmale: Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15 μg/mL in the presence of 2 ng/mL of rHuFas Ligand.

Reinheit: > 95 % by SDS-PAGE and HPLC analyses.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1 EU/μg of rHusFasR/TNFRSF6 as determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Buffer: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.

Antigendetails

Target: PTPN13 (Protein tyrosine Phosphatase, Non-Receptor Type 13 (APO-1/CD95 (Fas)-Associated Phosphatase) (PTPN13))

Abstract: PTPN13 Produkte

Synonyme: FAP-1 Protein, PNP1 Protein, PTP-BAS Protein, PTP-BL Protein, PTP1E Protein, PTPL1 Protein, PTPLE Protein, hPTP1E Protein, PTPN13 Protein, AI324989 Protein, Ptpri Protein, RIP Protein, Ptp-bl Protein, ptp Protein, wu:fb50g04 Protein, zgc:162319 Protein, protein tyrosine phosphatase, non-receptor type 13 Protein, tyrosine-protein phosphatase non-receptor type 13 Protein, PTPN13 Protein, LOC100540743 Protein, ptpn13 Protein, Ptpn13 Protein

Hintergrund: Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL.
Synonyms: soluble Fas receptor (sFasR), TNFRSF6, CD95, Apo I, Fas Antigen

Molekulargewicht: 17.6 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids.