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PDGF-BB Homodimer (AA 82-190) (Active) Protein

Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE analysis. Active
Produktnummer ABIN2018322
  • Target
    PDGF-BB Homodimer
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 82-190
    Spezies
    • 10
    • 4
    • 3
    Maus
    Quelle
    • 12
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Produktmerkmale
    ED50 < 2.5 ng/mL, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 4 x 10^5 units/mg.
    AA 82-190, expressed with an N-terminal Met.
    Reinheit
    > 95 % by SDS-PAGE analysis.
    Endotoxin-Niveau
    < 0.2 EU/μg, determined by LAL method.
  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstituted in ddH2O at 100 μg/mL.
    Buffer
    Lyophilized after extensive dialysis against 10 mM Sodium Citrate, pH 3.0.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Lyophilized recombinant mouse Platelet-Derived Growth Factor-BB (rmPDGF-BB) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmPDGF-BB remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
    Haltbarkeit
    6 months
  • Target
    PDGF-BB Homodimer
    Andere Bezeichnung
    Platelet-Derived Growth Factor-BB (PDGF-BB)
    Synonyme
    PDGF-2 Protein, PDGF2 Protein, SIS Protein, SSV Protein, c-sis Protein, PDGF-B Protein, Sis Protein, platelet derived growth factor subunit B Protein, platelet derived growth factor, B polypeptide Protein, PDGFB Protein, Pdgfb Protein
    Hintergrund
    Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, development of human skeleton, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.Recombinant mouse Platelet-Derived Growth Factor-BB (rmPDGF-BB) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 110 amino acids each. A fully biologically active molecule, rmPDGF-BB has a molecular mass of 24.7 kDa analyzed by non-reducing SDS-PAGE.
    Synonyms: PDGF-2, GDGF, ODGF, SIS, SSV
    Molekulargewicht
    24.7 kDa, observed by non-reducing SDS-PAGE.
    UniProt
    P31240
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