PDGF-BB Homodimer (Active) protein (His tag)
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- Target
- PDGF-BB Homodimer
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
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Spezies
- Maus
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- His tag
- Verwendungszweck
- Active Recombinant Mouse PDGF-BB Protein
- Sequenz
- SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TIVTPRPVT
- Spezifität
- Ser82-Thr190
- Reinheit
- > 97 % by SDS-PAGE.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- < 1EU/μg
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA.Immobilized Human PDGFB at 2 μg/mL (100 μL/well) can bind Human PDGFRB with a linear range of 12-153 ng/mL.
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PDGF-BB Homodimer (Active) protein (His tag)
Spezies: Human Wirt: Hefe Recombinant > 95 % as determined by reducing SDS-PAGE. Active
PDGF-BB Homodimer (AA 82-190) (Active) proteinSpezies: Human Wirt: Escherichia coli (E. coli) Recombinant >90 % as determined by reducing (R) and non-reducing (NR) SDS-PAGE. Active
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of 20 mMNaAc-Hac pH 4.5
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- Target
- PDGF-BB Homodimer
- Andere Bezeichnung
- PDGF-BB
- Synonyme
- PDGF-2 Protein, PDGF2 Protein, SIS Protein, SSV Protein, c-sis Protein, PDGF-B Protein, Sis Protein, platelet derived growth factor subunit B Protein, platelet derived growth factor, B polypeptide Protein, PDGFB Protein, Pdgfb Protein
- Hintergrund
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Description: PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialised mesenchymal and migratory cell types, both during development and in the adult animal. Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C)which are active in angiogenesis and endothelial cell growth, and placenta growth factor (PlGF) which is also active in angiogenesis. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. PDGF is a required element in cellular division for fibroblast, a type of connective tissue cell. PDGF is also known to maintain proliferation of oligodendrocyte progenitor cells. Platelet-derived growth factor subunit B is also known as PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis, is a member of the platelet-derived growth factor family. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.
Name: Sis, c-sis, PDGF-2, PDGF-B,PDGFB
- Gen-ID
- 18591
- UniProt
- P31240
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