M-CSF/CSF1 Protein (AA 33-190, Isoform 3)

Produktnummer ABIN2018212

Produktdetails

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: Isoform 3, AA 33-190

Spezies: Human

Quelle: Escherichia coli (E. coli)

Produktmerkmale: ED50 of 1 - 3 ng/mL, measured by cell proliferation assay of M-NFS-60, corresponding to a specific activity of 3.3 x 105-1 x 10^6 units/mg.
AA 33-190 (Isoform 3), expressed with an N-terminal Met.

Reinheit: > 95 % as analyzed by non-reducing SDS-PAGE.

Endotoxin-Niveau: < 1 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O or PBS or Tris-HCl, pH 8.0 at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant human Macrophage Colony-Stimulating Factor 1 (rhM-CSF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhM-CSF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

Haltbarkeit: 6 months

Referenzen

Bertin, Barat, Bélanger, Tremblay: "Leukotrienes inhibit early stages of HIV-1 infection in monocyte-derived microglia-like cells." in: Journal of neuroinflammation, Vol. 9, pp. 55, (2012) (PubMed).

Antigendetails

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Andere Bezeichnung: Macrophage Colony Stimulating Factor (M-CSF) (CSF1 Produkte)

Synonyme: CSF-1 Protein, MCSF Protein, C87615 Protein, Csfm Protein, op Protein, csf1-1 Protein, zgc:172186 Protein, CSF1 Protein, csf1-2 Protein, zgc:158436 Protein, colony stimulating factor 1 Protein, colony stimulating factor 1 (macrophage) Protein, colony stimulating factor 1a (macrophage) Protein, macrophage colony-stimulating factor 1 Protein, macrophage colony stimulating factor Protein, colony stimulating factor 1b (macrophage) Protein, CSF1 Protein, Csf1 Protein, csf1a Protein, LOC396599 Protein, LOC100860895 Protein, csf1b Protein

Hintergrund: Macrophage Colony-Stimulating Factor 1 (M-CSF), involved especially in monocytopoiesis,[1]is a hematopoietic growth factor. In mammals, it exits three isoforms, which invariably share an N-terminal 32-aa signal peptide, a 149-residue growth factor domain, a 21-residue transmembrane region and a 37-aa cytoplasmictail[2]. The biological activity of human M-CSF is maintained within the 149-aa growth factor domain[3], and it is only active in the disulfide-linked dimeric form[4], which is bonded at Cys63. Recombinant human Macrophage Colony-Stimulating Factor 1 (rhM-CSF) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 159 amino acids each. A fully biologically active molecule, rhM-CSF has a molecular mass of 28 kDaanalyzed by non-reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques.
Synonyms: Macrophage Colony Stimulating Factor, CSF-1, Lanimostim, MCSF, MGC31930, M-CSF.

Molekulargewicht: 28 kDa, observed by non-reducing SDS-PAGE.

UniProt: P09603

Pathways: RTK Signalweg